Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

4.0K Views

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05:38 min

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February 12th, 2021

DOI :

10.3791/62206-v

February 12th, 2021

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Shiqiao Ye¹^,², Xiaoping Wan³, Juan Su¹^,², Akshar Patel¹^,²^,⁴, Blake Justis¹^,²^,⁵, Isabelle Deschênes³, Ming-Tao Zhao¹^,²^,⁵^,⁶

¹Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, ²The Heart Center, Nationwide Children’s Hospital, ³Department of Physiology and Cell Biology, The Ohio State University College of Medicine, ⁴Department of Anatomy, The Ohio State University College of Medicine, ⁵MCDB Graduate Program, The Ohio State University, ⁶Department of Pediatrics, The Ohio State University College of Medicine

Summary

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Human Cardiomyocytes

Patient Peripheral Blood Mononuclear Cells

Stem Cell Reprogramming

Cardiac Toxicity Testing

Cardiomyocyte Differentiation

IPSC Colonies

DPBS

Cardiomyocyte Differentiation Medium

Culture Confluency

Transfection

Sendai Virus Reprogramming Factors

Chapters in this video

0:04

Introduction

1:08

Human Induced Pluripotent Stem Cell (iPSC) Maintenance and Passaging

1:55

Chemically Defined Cardiomyocyte (CM) Differentiation

2:57

Results: Representative iPSC-CM Differentiation and Function

4:51

Conclusion

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