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A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

DOI :

10.3791/62354-v

April 15th, 2021

April 15th, 2021

5,373 Views

1Florida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, 2U. S. Fish and Wildlife Service, Pacific Islands Fish and Wildlife Office, 3Department of Entomology and Nematology, University of California Davis

Described here is a DNA extraction protocol using magnetic beads to produce high quality DNA extractions from mosquitoes. These extractions are suitable for a downstream next-generation sequencing approach.

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