Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

25.6K Views

06:38 min

August 2nd, 2021

DOI :

10.3791/62652-v

August 2nd, 2021


Explore More Videos

Macrophage Differentiation

Chapters in this video

0:05

Introduction

0:56

Culturing and Maintenance of THP‐1 Monocyte-like Cells

2:59

Seeding of THP‐1 cells and Differentiation into M0 Macrophages

4:01

Polarization of M0 Macrophages into M2‐like Macrophages

4:39

Detaching and Harvesting Macrophages for Flow Cytometry

5:14

Results: Macrophage Differentiation and Polarization into an M2‐Like Phenotype

5:56

Conclusion

Related Videos

article

12:22

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

22.1K Views

article

10:07

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

65.7K Views

article

07:42

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

18.6K Views

article

13:47

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

11.5K Views

article

08:11

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

8.6K Views

article

07:54

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

46.4K Views

article

07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

51.5K Views

article

09:14

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

10.1K Views

article

11:17

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

11.8K Views

article

06:35

An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

7.0K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved