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Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

26.2K Views

06:38 min

August 2nd, 2021

DOI :

10.3791/62652-v

August 2nd, 2021


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Macrophage Differentiation

Chapters in this video

0:05

Introduction

0:56

Culturing and Maintenance of THP‐1 Monocyte-like Cells

2:59

Seeding of THP‐1 cells and Differentiation into M0 Macrophages

4:01

Polarization of M0 Macrophages into M2‐like Macrophages

4:39

Detaching and Harvesting Macrophages for Flow Cytometry

5:14

Results: Macrophage Differentiation and Polarization into an M2‐Like Phenotype

5:56

Conclusion

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