Dot Blot to Show the Presence of dsRNA Structures in HDM Total RNA
3:54
FACS Analysis to Determine the Effects of HDM RNA on the Infiltration of Eosinophils in BAL and Lung
6:19
Results: dsRNA Structures in HDM RNAs
7:53
Conclusion
Transcript
This protocol was designed to address a very important and unsolved issue on how microbial substances present and environmental allergens can regulate the development of allergic inflammation. Specifically, the methods presented here can help answ
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Environmental allergens such as house dust mites (HDM) often contain microbial substances that activate innate immune responses to regulate allergic inflammation. The protocol presented here demonstrates the identification of dsRNA species in HDM allergens and characterization of their immunogenic activities in modulating eosinophilic lung inflammation.