Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

3.6K Views

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14:56 min

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May 20th, 2022

DOI :

10.3791/62803-v

May 20th, 2022

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Sophie Sosnovskaya*¹^,², Amir N. Zakirov*¹^,², Ekaterina D. Ryumina*¹^,³, Ekaterina Kharybina¹^,², Sergei A. Golyshev¹, Olga S. Strelkova¹, Oxana A. Zhironkina¹, Andrei Moiseenko², Anton Orekhov⁴, Igor I. Kireev¹^,²^,⁵

¹Belozersky Institute of Physico-chemical Biology, Lomonosov Moscow State University, ²Faculty of Biology, Lomonosov Moscow State University, ³Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, ⁴National Research Center, Kurchatov Institute, ⁵V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology

Summary

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Electron Tomography

Replicative Domains

Chromatin Visualization

EdU Labeling

Nanogold Particles

Silver Enhancement

Glutaraldehyde Fixation

Structural Preservation

Streptavidin Nanogold

Moist Chamber Protocol

Chapters in this video

0:08

Introduction

0:35

Cells Labeling and Fixation

2:13

Click-Reaction

5:09

Ag-Amplification

6:28

Gold Toning

6:42

ChromEM

7:23

Dehydration and Epon Embedding

12:50

Electron Tomography

13:06

Representative Results

14:14

Conclusions

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