A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma

5.0K Views

•

12:24 min

•

September 30th, 2021

DOI :

10.3791/62868-v

September 30th, 2021

•

Marco Y. W. Zaki*¹^,², Shishir Shetty*², Alex L. Wilkinson², Daniel A. Patten², Fiona Oakley*³, Helen Reeves*⁴^,⁵

¹Department of Biochemistry, Faculty of Pharmacy, Minia University, ²National Institute for Health Research Birmingham Liver Biomedical Research Unit and Centre for Liver and Gastrointestinal Research, Institute of Immunology and Immunotherapy, University of Birmingham, ³Newcastle Fibrosis Research Group, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, ⁴Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, ⁵The Liver Unit, Department of Medicine, Freeman Hospital, Newcastle-upon-Tyne Hospitals NHS Foundation Trust

Summary

Explore More Videos

Three dimensional Spheroid

Tumor stromal Interaction

Hepatocellular Carcinoma

2D Monolayer

Co culture

Tumor Microenvironment

Hanging Droplets Method

COS 7 Fibroblast Line

Chapters in this video

0:05

Introduction

1:20

Cell Preparation

2:17

Cell Collection and Counting

4:14

Collection of Fibroblast Conditioned Media

5:17

Heterotypic Tumor/Stromal Spheroids

6:29

Homotypic Hep3B Spheroids in LX2 Conditioned Media

8:18

Calculation of Spheroid Volume

9:17

Results: In Vitro Creation and Culture of 3D Tumor Spheroid Model

11:28

Conclusion

Related Videos

09:13

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

8.0K Views

06:11

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

9.7K Views

08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

7.1K Views

08:15

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

15.4K Views

07:20

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

9.8K Views

07:40

A 3D Spheroid Model for Glioblastoma

14.6K Views

05:31

Transradial Access Chemoembolization for Hepatocellular Carcinoma Patients

5.3K Views

06:07

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

5.5K Views

06:38

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

5.8K Views

06:35

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

4.3K Views