Free-Floating Wisteria Florbunda Agglutinin Staining and Anti- Arginine Vasopressin Immunostaining
5:13
Mounting
6:00
Imaging
6:42
Results: Double Immunofluorescent Staining of Free-Floating Mouse Brain Sections
7:21
Conclusion
Transkript
Immuno-histochemical staining of mouse brains is a commonly-used technique in neuroscience research. However, the quality, reliability, and reproducibility of brain histology results may vary among different researchers and laboratories. We presen
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This protocol describes an efficient and reproducible approach for mouse brain histological studies, including perfusion, brain sectioning, free-floating immunostaining, tissue mounting, and imaging.