Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

31.4K Views

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10:40 min

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October 27th, 2019

DOI :

10.3791/60510-v

October 27th, 2019

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Fernando González Ibanez¹^,², Katherine Picard¹^,², Maude Bordeleau¹^,³, Kaushik Sharma¹^,²^,⁴, Kanchan Bisht¹^,²^,⁴, Marie-Ève Tremblay¹^,²

¹Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, ²Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, ³Integrated Program in Neuroscience, McGill University, ⁴Center for Brain Immunology and Glia (BIG), University of Virginia

Summary

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Immunofluorescence Staining

Microglial Density

Microglial Morphology

Macrophage Infiltration

Mouse Brain Analysis

Quantitative Analysis

Anti IBA1 Antibodies

Anti TMEM119 Antibodies

Image Processing Program

Nearest Neighbor Distance

Area Measurement

Chapters in this video

0:04

Title

1:00

Density and Distribution Analysis

5:50

Morphology Analysis

9:01

Results: Immunofluorescence Staining Using IBA1 and TMEM119

10:06

Conclusion

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