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Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

DOI :

10.3791/63213-v

8:26 min

January 13th, 2022

January 13th, 2022

3,915 Views

1School of Applied and Engineering Physics, Cornell University, 2Department of Neurobiology and Behavior, Cornell University, 3College of Veterinary Medicine, Cornell University, 4Meinig School of Biomedical Engineering, Cornell University

Three-photon microscopy enables high-contrast fluorescence imaging deep in living biological tissues, such as mouse and zebrafish brains, with high spatiotemporal resolution.

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Deep tissue Three photon Fluorescence Microscopy

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