Zebrafish Maintenance and Pre-ICV Microinjection Preparations
2:19
Anaesthetisation and ICV Injection of Zebrafish
4:10
Locomotor Assessment
5:22
Results: Assessment of Neuroregeneration in Zebrafish Based PD Model
6:44
Conclusion
Transcript
The protocol is significant in demonstrating zebrafish motor impairment and a subsequent recovery, following intracerebroventricular injection of neurotoxin 6-hydroxydopamine at the ventral diencephalon. This technique demonstrates changes in the
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The present protocol describes the intracerebroventricular (ICV) injection of adult zebrafish with neurotoxic 6-hydroxydopamine (6-OHDA) at the ventral diencephalon (Dn) and the assessment of the impairment and subsequent recovery of swimming behavior postlesion by using the open tank test, which is accompanied by analysis using a video tracking software.