Isolation and Homogenization of Mouse Tissues to Quantify Microtubule Fractions
1:53
Fractionation of Mouse Tissues
4:04
Results: Analysis of the Characterized Murine Microtubule Brain Fractions
6:25
Conclusion
Transcript
Our new protocol helps to distinguish and quantify three statuses of tube rings, such as stable microtubules, labile microtubules, and free tubules in animal tissues. This technique is composed of simple steps and can evaluate the structural stabi
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Microtubules, which are tubulin polymers, play a crucial role as a cytoskeleton component in eukaryotic cells and are known for their dynamic instability. This study developed a method for fractionating microtubules to separate them into stable microtubules, labile microtubules, and free tubulin to evaluate the stability of microtubules in various mouse tissues.