JoVE Journal
Biology
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A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay
4.4K Views
•
05:53 min
•
December 7th, 2021
DOI :
December 7th, 2021
•Chapters in this video
0:05
Introduction
0:36
Plating and Infecting HT1080 Cells
2:00
Day 3: Fixing and Adding Substrate to The Cells
3:04
Quantitation to Determine the Neutralizing Activity Using ImageJ
3:48
Results: Transduction Assay and Assessment of Neutralizing Antibodies (Nabs) Against AAV6 in Sheep Samples
5:26
Conclusion
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