Trimming and Serial Sectioning of Embedded Samples
4:12
Grid Staining
5:26
Imaging Acquisition by TEM
6:52
Segmentation and 3D Reconstruction
7:31
Results: Segmentation and 3D Reconstruction Reviewing the Morphology of Endoplasmic Reticulum and the Associated ER-Mitochondria Contacts
8:34
Conclusion
Transcript
This protocol allows 3D reconstruction of organelles in a simple and robust manner, whilst being accessible to laboratories that do not currently have specialist volume electron microscopes. This technique is extremely flexible, providing excellen
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A simple and comprehensive protocol to acquire three-dimensional details of membrane contact sites between organelles in hepatocytes from the liver or cells in other tissues.