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Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

5.3K Views

09:12 min

June 9th, 2022

June 9th, 2022

5,252 Views

0:05

Introduction

1:08

Trimming and Serial Sectioning of Embedded Samples

4:12

Grid Staining

5:26

Imaging Acquisition by TEM

6:52

Segmentation and 3D Reconstruction

7:31

Results: Segmentation and 3D Reconstruction Reviewing the Morphology of Endoplasmic Reticulum and the Associated ER-Mitochondria Contacts

8:34

Conclusion

Transcript

This protocol allows 3D reconstruction of organelles in a simple and robust manner, whilst being accessible to laboratories that do not currently have specialist volume electron microscopes. This technique is extremely flexible, providing excellen

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A simple and comprehensive protocol to acquire three-dimensional details of membrane contact sites between organelles in hepatocytes from the liver or cells in other tissues.

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