April 11th, 2022
•MALDI-TOF was used to characterize fragments obtained from the reactivity between oxidized RNA and the exoribonuclease Xrn-1. The present protocol describes a methodology that can be applied to other processes involving RNA and/or DNA.
Related Videos
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
Nitropeptide Profiling and Identification Illustrated by Angiotensin II
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved