Separation of Multi-Layered 2D Crystals into Single Layer
4:55
Results: 2D Crystals of Human Leukotriene C4 Synthase and Liposomes Subjected to the Peel‐Blot Method, the Peel-Blot Footprint, and Blotting an EM Grid Prepared by Back-Injection on a Submicron Filter Paper
6:42
Conclusion
Transcript
The peel-blot technique allows for the separation of multi-layered and concentrated biological cryo-EM samples into single layers to reduce thickness, increase concentration, and facilitate image processing. Sample concentrations can be increased
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The peel-blot technique is a cryo-EM grid preparation method that allows for the separation of multilayered and concentrated biological samples into single layers to reduce thickness, increase sample concentration, and facilitate image processing.