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The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples
2.2K Views
•
07:27 min
•
June 29th, 2022
DOI :
June 29th, 2022
•Chapters in this video
0:04
Introduction
0:33
Preparation Steps for the Peel‐Blot Technique
1:57
Placing the Carbon Film onto the Grid
3:07
Separation of Multi-Layered 2D Crystals into Single Layer
4:55
Results: 2D Crystals of Human Leukotriene C4 Synthase and Liposomes Subjected to the Peel‐Blot Method, the Peel-Blot Footprint, and Blotting an EM Grid Prepared by Back-Injection on a Submicron Filter Paper
6:42
Conclusion
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