A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

3.8K Views

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05:26 min

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September 16th, 2022

DOI :

10.3791/64123-v

September 16th, 2022

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Julie Earl¹, Silvia Calabuig-Fariñas¹, María Eugenia Sarasquete¹, Laura Muinelo Romay¹, Sara Lopez-Tarruella¹, Beatriz Bellosillo Paricio¹, Marta Rodríguez¹, Karmele Valencia Leoz¹, Marta Dueñas Porto¹, Noelia Tarazona¹, Javier Hernandez Losa¹, Rodrigo Almeida Toledo¹

¹The Liquid Biopsy and Biomarker Working Module, the Biomedical Research Network in Cancer (CIBERONC)

Summary

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Liquid Biopsy

Standardized Protocol

Circulating Free DNA

Blood Sample Processing

Oncology Research

Clinical Applications

Preanalytical Protocol

Laboratory Procedures

Aliquots Labeling

Sample Freezing

Plasma Processing

Chapters in this video

0:05

Introduction

1:30

Serum Preparation for Liquid Biopsy Studies

2:25

Plasma Preparation for Liquid Biopsy Studies

4:08

Results: Electrophoresis-Based Analysis of cfDNA Isolated from Plasma

4:28

Conclusion

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