Cell-Free Protein Synthesis from Exonuclease-Deficient Cellular Extracts Utilizing Linear DNA Templates
3.5K Views
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08:11 min
•
August 9th, 2022
DOI :
August 9th, 2022
•Mahnaz Sabeti Azad*1, Angelo Cardoso Batista*1, Jean-Loup Faulon1, Chase L. Beisel2,3, Jerome Bonnet4, Manish Kushwaha1
1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier
Chapters in this video
0:05
Introduction
0:50
Cell Culture and Lysate Preparation
4:17
Cell-Free Buffer Calibration for Linear DNA
5:49
Experimental Execution
6:33
Results: Expression in Cell-Free Protein Synthesis Systems Using Linear DNA Templates
7:26
Conclusion
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