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Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi

1.9K Views

09:01 min

September 28th, 2022

DOI :

10.3791/64408-v

September 28th, 2022


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Phage mediated Genetic Manipulation

Chapters in this video

0:04

Introduction

0:40

Preparation of Borrelia burgdorferi Culture for the Production of φBB-1 and Induction of Borrelia burgdorferi Phage φBB-1

2:31

Polyethylene Glycol Precipitation to Recover Phage for Use in Transduction Assay

5:34

Transduction Assay Following Polyethylene Glycol Precipitation of φBB-1 and Selection of Transductants

6:51

Results: Transducing Heterologous DNA Between Different Strains of the Lyme Disease Spirochete Using Borrelia burgdorferi Bacteriophage

8:12

Conclusion

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