Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples
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10:13 min
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December 2nd, 2022
DOI :
December 2nd, 2022
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Chapters in this video
0:04
Introduction
0:51
Preparation of Functionalized Slides
1:34
Generation of Closed Circular Substrates
2:16
Blocking of the Wellmaker and Hybridization of the Circles
3:48
Rolling Circle Amplification (RCA) and Visualization by Fluorescence Microscopy
5:54
Coupling of HRP-Conjugated Antibody to Rolling Circle Products and Visualization
7:57
Results: Detection of Topoisomerase 1 Activity Using the REEAD Assay
9:29
Conclusion
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