Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions
1.6K Views
•
07:25 min
•
December 2nd, 2022
DOI :
December 2nd, 2022
•Chapters in this video
0:05
Introduction
0:49
Prepare Palmitic Acid Working Solution
1:27
Applying Palmitic Acid Coating to Microtray
2:53
Loading the Microtray with Low‐Melt Nematode Growth Media (lmNGM) and Seeding the Microtray Wells with Bacterial Food
3:47
Enclosing Each Microtray Inside a Single‐Well Plate
4:25
Adding Worms to the Microtray and Preparing the Culture Environment for Long‐Term Use
5:01
Imaging Individual Worms in Microtray Wells
5:27
Results: Correlation of Phenotypes in Individual Animals Across Populations
6:53
Conclusion
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