Loading the Microtray with Low‐Melt Nematode Growth Media (lmNGM) and Seeding the Microtray Wells with Bacterial Food
3:47
Enclosing Each Microtray Inside a Single‐Well Plate
4:25
Adding Worms to the Microtray and Preparing the Culture Environment for Long‐Term Use
5:01
Imaging Individual Worms in Microtray Wells
5:27
Results: Correlation of Phenotypes in Individual Animals Across Populations
6:53
Conclusion
필기록
While other solid culture methods exist, this is the first solid culture system that allows automated longitudinal tracking of individual animal life and health span and longitudinal imaging of fluorescent biomarkers in one system. This protocol p
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Here, we present a protocol to culture isolated individual nematodes on solid media for lifelong physiological parameter tracking and fluorescence quantification. This culture system includes a palmitic acid barrier around single-worm wells to prevent animals from fleeing, allowing the use of aversive interventions, including pathogenic bacteria and chemical stressors.