Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology

4.9K Views

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10:26 min

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August 18th, 2023

DOI :

10.3791/65414-v

August 18th, 2023

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Criselda Bautista¹^,², Gurdeep Jaswant¹^,³^,⁴^,⁵, Hollie French¹^,⁶, Kathryn Campbell¹, Rowan Durrant¹, Robert Gifford¹^,⁶, Grace S. N. Kia⁷^,⁸, Brian Ogoti³^,⁹, Katie Hampson¹, Kirstyn Brunker¹^,⁶

¹School of Biodiversity, One Health & Veterinary Medicine, University of Glasgow, ²Research Institute for Tropical Medicine, ³University of Nairobi, Institute of Tropical and Infectious Diseases, ⁴Tanzania Industrial Research Development Organization, ⁵Ifakara Health Institute, ⁶MRC-University of Glasgow Centre for Virus Research, ⁷Department of Veterinary Public health, Ahmadu Bello University, ⁸African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, ⁹University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis, University of Nairobi

Summary

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Whole Genome Sequencing

Rabies Virus

Nanopore Technology

Pathogen Surveillance

Low income Countries

Genomic Data

Bioinformatic Tools

Viral Genomic Surveillance

Rabies Elimination Strategies

Infectious Diseases

Sample to sequence Workflow

Decentralize Sequencing

Data Interpretation

Public Health Concerns

Epidemiological Insight

Chapters in this video

0:00

Introduction

2:38

Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV)