AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

379 Views

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06:03 min

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June 23rd, 2023

DOI :

10.3791/64857-v

June 23rd, 2023

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Rafael Badain¹, Daniel S. C. Damineli¹^,², Maria Teresa Portes³, José Feijó⁴, Stefano Buratti⁵, Giorgia Tortora⁶, Hugo Neves de Oliveira¹, Roberto M. Cesar Jr¹

¹Institute of Mathematics and Statistics, University of São Paulo, ²Center for Mathematics, Computing and Cognition, Federal University of ABC, ³Department of Botany, Institute of Biosciences, University of São Paulo, ⁴Cell Biology and Molecular Genetics Department, University of Maryland, ⁵Department of Biosciences, University of Milan, ⁶Department of Physics, Politecnico di Milano

Summary

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AMEBaS

Automatic Midline Extraction

Background Subtraction

Ratiometric Fluorescence

Polarized Single Cells

Spatiotemporal Dynamics

Single Cell Segmentation

Segmentation Mask

Sigma Value

Local Polynomial Regression

Midline Tracing

Lee s Method

Skeletonization

Chapters in this video

0:04

Introduction

0:35

Interactive Notebook Protocol

4:52

Results: Analyzing the Different Fluorescence Image Datasets

5:18

Conclusion

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