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Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

DOI :

10.3791/61086-v

8:37 min

October 9th, 2020

October 9th, 2020

4,923 Views

1Development, Disease Models & Therapeutics Graduate Program, Department of Molecular and Cellular Biology, Department of Neuroscience, Center for Cell and Gene Therapy, Stem Cells and Regenerative Medicine Center, Baylor College of Medicine

We describe a technique for profiling microRNAs in early mouse embryos. This protocol overcomes the challenge of low cell input and small RNA enrichment. This assay can be used to analyze changes in miRNA expression over time in different cell lineages of the early mouse embryo.

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Small RNA Libraries

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