Micropatterning is a powerful technique employed by scientists to understand connections between cell shape and the activity of intercellular machines. Although several techniques allow researchers to modulate cell shape, these techniques often re
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The protocol presented here enables automated fabrication of micropatterns that standardizes cell shape to study cytoskeletal structures within mammalian cells. This user-friendly technique can be set up with commercially available imaging systems and does not require specialized equipment inaccessible to standard cell biology laboratories.
