Preparation of Plants for Glass Bottom Dishes and 3-D Image Reconstruction
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Results: 3-D Time Series of Moss Development
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Conclusion
副本
Time-lapse imaging with fluorescence microscopy allows observation of the dynamic changes of growth and development at cellular and subcellular levels. This simple protocol for 3D time-lapse imaging can directly study cell wall dynamics over a lon
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This manuscript presents a detailed protocol to image the 3-D cell wall dynamics of living moss tissue, allowing the visualization of the detachment of cell walls in ggb mutants and thickening cell wall patterns in the wild type during development over a long period.