Transsynaptische Tracing von Peripherie Targets mit Pseudorabies-Virus durch Cholera Toxin und biotinyliertes Dextran Amine Doppelmarkierung Gefolgt

Transsynaptic tracing has become a powerful tool for analyzing central efferents regulating peripheral targets through multi-synaptic circuits. Here we present a protocol that exploits the transsynaptic pseudorabies virus to identify and localize a functional brain circuit, followed by classical tract tracing techniques to validate specific connections in the circuit between identified groups of neurons.