Gene Expression Assay Preparation and Surface Staining of Viable Cells
3:18
Prepare Cell Collection Plates, Perform FACS Sort, and Generate cDNA
5:17
Multiplex qPCR on the Biomark Platform
11:26
Results: Single-cell Quantitation of mRNA and Surface Protein Expression
12:56
Conclusion
Transkript
This method can help answer key questions in the field of host-pathogen interactions such as what are the unique characteristics of the productively infected cells, what host cofactors allow viruses to establish productive infection, and how to ta
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Described is a methodology to quantitate the expression of 96 genes and 18 surface proteins by single cells ex vivo, allowing for the identification of differentially expressed genes and proteins in virus-infected cells relative to uninfected cells. We apply the approach to study SIV-infected CD4+ T cells isolated from rhesus macaques.