Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

10.3K Views

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07:00 min

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October 8th, 2018

DOI :

10.3791/58299-v

October 8th, 2018

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10,269 Views

Hanna Luukinen¹, Milka Marjut Hammarén¹, Leena-Maija Vanha-aho¹, Mataleena Parikka^1,2

¹Faculty of Medicine and Life Sciences, University of Tampere, ²Oral and Maxillofacial Unit, Tampere University Hospital

Kapitel

0:04

Title

0:37

Culturing of Mycobacterium marinum and Preparation of Bacterial Solution for Infecting Adult Zebrafish

2:10

Experimental M. marinum Infection with Intraperitoneal Injection

3:32

Collection of Internal Organs

4:36

Results: Mycobacterium marinum Infection Modeling

6:02

Conclusion

Transkript

This method can help to answer key questions in the field of tuberculosis such as what is the role of adaptive immune responses and the pathogenesis of this multifactorial disease. The main advantage of this nucleic acid base method is that both b

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Zusammenfassung

Here, we present a protocol to model human tuberculosis in an adult zebrafish using its natural pathogen Mycobacterium marinum. Extracted DNA and RNA from the internal organs of infected zebrafish can be used to reveal the total mycobacterial loads in the fish and the host's immune responses with qPCR.

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Keywords Mycobacterium Marinum

Tuberculosis

Zebrafish

Adaptive Immune Responses