Preparing the Crystalline Silica Dust (CSD) Suspension
1:52
Administering the Nasal Drips to the Mouse
2:24
Performing Hematoxylin and Eosin (HE) Staining
3:23
Performing Masson Staining and Sirius Red Staining
4:27
Performing Immunohistochemistry
6:10
Performing Western Blotting Analysis
8:02
Results: Investigating the Development of Silicosis in Mice After Repeated CSD Inhalation
9:41
Conclusion
Transkript
In the described mouse silicosis model generated by intra laser infusion of crystal silica dust, all CSD suspension several times simulated the occurrence and development of human silicosis to a large extent. This method is time saving and level s
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This protocol describes a method for establishing a mouse model of silicosis through repeated exposure to silica suspensions via a nasal drip. This model can efficiently, conveniently, and flexibly mimic the pathological process of human silicosis with high repeatability and economy.