Diese Arbeit wurde finanziell unterstützt von der National Natural Science Foundation of China (Nr. 82130113), der China Postdoctoral Science Foundation (Nr. 2021MD703800), der Science Foundation for Youths of Science & Technology Department der Provinz Sichuan (Nr. 2022NSFSC1449) und dem Forschungsförderungsprogramm "Xinglin Scholars" der Chengdu University of Traditional Chinese Medicine (Nr. 2022NSFSC1449) BSH2021009).

Eine Methode zur Prozessoptimierung von Zanba-Pfannengerichten mit Tiebangchui

<ol>
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S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetically+levitated+mesenchymal+stem+cell+spheroids+cultured+with+a+collagen+gel+maintain+phenotype+and+quiescence.">Magnetically levitated mesenchymal stem cell spheroids cultured with a collagen gel maintain phenotype and quiescence.</a> Journal of Tissue Engineering. 8, (2017).</li><li>Chinese Pharmacopoeia Committee. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Pharmacopoeia of the People's Republic of China. 4, (2020).</li><li>Li, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+response+surface+methodology-optimized+ultrasound-assisted+pretreatment+extraction+on+the+composition+of+essential+oil+released+from+tribute+citrus+peels.">Effect of response surface methodology-optimized ultrasound-assisted pretreatment extraction on the composition of essential oil released from tribute citrus peels.</a> Frontiers in Nutrition. 9, 840780 (2022).</li><li>Liu, X. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hezi+inhibits+Tiebangchui-induced+cardiotoxicity+and+preserves+its+anti-rheumatoid+arthritis+effects+by+regulating+the+pharmacokinetics+of+aconitine+and+deoxyaconitine.">Hezi inhibits Tiebangchui-induced cardiotoxicity and preserves its anti-rheumatoid arthritis effects by regulating the pharmacokinetics of aconitine and deoxyaconitine.</a> Journal of Ethnopharmacology. 302, 115915 (2023).</li><li>Smolen, J. 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D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Online+discovery+of+the+molecular+mechanism+for+directionally+detoxification+of+Fuzi+using+real-time+extractive+electrospray+ionization+mass+spectrometry.">Online discovery of the molecular mechanism for directionally detoxification of Fuzi using real-time extractive electrospray ionization mass spectrometry.</a> Journal of Ethnopharmacology. 277, 114216 (2021).</li><li>El-Shazly, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use,+history,+and+liquid+chromatography/mass+spectrometry+chemical+analysis+of+Aconitum.">Use, history, and liquid chromatography/mass spectrometry chemical analysis of Aconitum.</a> Journal of Food and Drug Analysis. 24 (1), 29-45 (2016).</li><li>Chan, T. Y. 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Die Autoren haben keine Interessenkonflikte offenzulegen.

TBC ist ein wichtiges tibetisches Arzneimittel mit der Wirkung, Kälte zu vertreiben und Schmerzen zu lindern. Es wird in China seit Tausenden von Jahren hauptsächlich zur Behandlung von traumatischen Verletzungen und rheumatischer Arthralgie eingesetzt24,25,26. Diterpenoidalkaloide sind sowohl aktive als auch toxische Bestandteile von TBC27,28,29<sup class="...

Die getrocknete Wurzel von Aconitum pendulum Busch und A. flavum Hand.-Mazz., eine der bekanntesten tibetischen Arzneimittel, wird auf Chinesisch Tiebangchui (TBC) genannt 1,2. Die getrockneten Wurzeln von TBC sind hilfreich, um Kälte und Wind zu vertreiben, Schmerzen zu lindern und Schock zu lindern. Es wurde im ersten Band der "Arzneimittelstandards (Tibetische Medizin) des Gesundheitsministeriums der Volksrepublik China" festgehalten, in dem es heißt, dass die getrockneten Wurzeln von TBC häufig zur Behandlung von rheumatoider Arthritis, Prellungen und anderen Erkältungskrankheiten verwendet werden3. Die klinische therapeutische Dosis von TBC ist jedoch ähnlich wie die toxische Dosis, und es wurde häufig über Vergiftungen oder Todesfälle aufgrund unsachgemäßer Anwendung berichtet4. Daher ist die Verringerung der Toxizität und die Erhaltung der Wirksamkeit von TBC im Laufe der Jahre zu einem Forschungsschwerpunkt geworden.
In der tibetischen Medizin ist die Verarbeitung eine der effektivsten Methoden, um die Toxizität von TBC abzuschwächen. Gemäß "Verarbeitungsspezifikation der tibetischen Medizin der Provinz Qinghai (2010)" sollten die Originalkräuter (TBC) in einen Eisentopf gegeben und mit Zanba gebraten werden, bis die Zanba gelb wird, danach wird Zanba entfernt und die Kräuter an der Luft getrocknet 5,6. Es wurden jedoch keine spezifischen Prozessparameter dokumentiert, was die Kontrolle der Verarbeitungstechnologie und der Qualität von Zanba-Pfannengerichten erschwert. Die CRITIC-Methode ist eine objektive Gewichtungsmethode, die Unschärfe und Subjektivität vermeiden und die Objektivität des Wiegens von7 verbessern kann. Die Box-Behnken-Antwortflächenmethode kann die Wechselwirkung zwischen den einzelnen Faktoren durch Polynomanpassung8 direkt widerspiegeln. Die Kombination der Box-Behnken-Antwortfläche und der CRITIC-Methode wird häufig verwendet, um die Verarbeitungstechnologie zu optimieren, um das optimierte Verarbeitungsprotokoll 9,10 zu erhalten. In dieser Arbeit wurden ein Monoester-Diterpenoid-Alkaloid (MDA) (Benzoylaconitin) und zwei Diester-Diterpenoid-Alkaloide (DDAs) (Aconitin, 3-Desoxyaconitin) als Bewertungsindizes verwendet. CRITIC in Kombination mit der Box-Behnken-Response-Surface-Methode wurde angewendet, um die Verarbeitungstechnologie von Zanba-gerührtem TBC zu optimieren und eine Standardverarbeitungsmethode für den klinisch sicheren Einsatz zu etablieren.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>3-Deoxyaconitine</td>
			<td>Chengdu Desite Biotechnology Co., Ltd.</td>
			<td>DST221109-033</td>
			<td></td>
		</tr>
		<tr>
			<td>Aconitine</td>
			<td>Chengdu Desite Biotechnology Co., Ltd.</td>
			<td>DSTDW000602</td>
			<td></td>
		</tr>
		<tr>
			<td>Ammonium acetate</td>
			<td>Tianjin Kermel Chemical Reagent Co., Ltd</td>
			<td>Chromatographic grade</td>
			<td></td>
		</tr>
		<tr>
			<td>Benzoylaconitine</td>
			<td>Chengdu Desite Biotechnology Co., Ltd.</td>
			<td>DSTDB005502</td>
			<td></td>
		</tr>
		<tr>
			<td>Design-Expert software</td>
			<td>Stat-Ease, Inc., Minneapolis, MN, USA</td>
			<td>version 13.0</td>
			<td></td>
		</tr>
		<tr>
			<td>Electronic analytical balance</td>
			<td>Shanghai Liangping Instruments Co., Ltd.</td>
			<td>FA1004</td>
			<td></td>
		</tr>
		<tr>
			<td>High performance liquid chromatography</td>
			<td>SHIMADZU Co., Ltd.</td>
			<td>LC-20A</td>
			<td></td>
		</tr>
		<tr>
			<td>High-speed smashing machine</td>
			<td>Beijing Zhongxing Weiye Instrument Co., Ltd.</td>
			<td>FW-100</td>
			<td></td>
		</tr>
		<tr>
			<td>Millipore filter</td>
			<td>Tianjin Jinteng Experimental Equipment Co., Ltd</td>
			<td>&#966;13 0.22 Nylon66</td>
			<td></td>
		</tr>
		<tr>
			<td>stir-Fry machine</td>
			<td>Changzhou Maisi Machinery Co., Ltd</td>
			<td>Type 5</td>
			<td></td>
		</tr>
		<tr>
			<td>Tiebangchui</td>
			<td>Gannan Baicao Biotechnology Development Co., Ltd</td>
			<td>20211012</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultra pure water systemic</td>
			<td>RephiLe Bioscience, Ltd.</td>
			<td>Genie G</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultrasonic cleansing machine</td>
			<td>Ningbo Xinyi Ultrasonic Equipment Co., Ltd</td>
			<td>SB2200</td>
			<td></td>
		</tr>
		<tr>
			<td>Zanba</td>
			<td>27 Chuanzang Road, Ganzi County</td>
			<td>-</td>
			<td></td>
		</tr>
	</tbody>
</table>

optimization of processing technology for tiebangchui with zanba based on critic combined with box-behnken response surface method

Die getrocknete Wurzel von Aconitum pendulum Busch., auf Chinesisch Tiebangchui (TBC) genannt, ist eine der bekanntesten tibetischen Arzneimittel. Es ist ein weit verbreitetes Kraut im Nordwesten Chinas. Viele Fälle von Vergiftungen sind jedoch aufgrund der intensiven Toxizität von TBC und der Tatsache, dass seine therapeutischen und toxischen Dosen ähnlich sind, aufgetreten. Daher ist es eine dringende Aufgabe, eine sichere und wirksame Methode zur Verringerung der Toxizität zu finden. Eine Recherche in den Klassikern der tibetischen Medizin zeigt, dass die Verarbeitungsmethode von TBC, die mit Zanba gebraten wird, in der "Verarbeitungsspezifikation der tibetischen Medizin der Provinz Qinghai" (2010) festgehalten wurde. Die konkreten Verarbeitungsparameter sind jedoch noch nicht klar. Ziel dieser Studie ist es daher, die Verarbeitungstechnologie von Zanba-Pfannengerichten zu optimieren und zu standardisieren.
Zunächst wurde ein Ein-Faktor-Experiment zu vier Faktoren durchgeführt: der Schichtdicke von TBC, der Menge an Zanba, der Verarbeitungstemperatur und der Zeit. Mit Monoester- und Diesteralkaloidgehalten in Zanba-gerührtem TBC als Indizes wurde CRITIC in Kombination mit der Box-Behnken-Response-Surface-Methode verwendet, um die Verarbeitungstechnologie von Zanba-gerührtem TBC zu optimieren. Die optimierten Verarbeitungsbedingungen von Zanba-Pfannengerichten waren eine TBC-Scheibendicke von 2 cm, dreimal mehr Zanba als TBC, eine Verarbeitungstemperatur von 125 °C und 60 Minuten Pfannengericht. Diese Studie ermittelte die optimierten und standardisierten Verarbeitungsbedingungen für die Verwendung von Zanba-Pfannen-TBC und lieferte damit eine experimentelle Grundlage für die sichere klinische Anwendung und industrielle Herstellung von Zanba-Pfannen-TBC.

The dried root of Aconitum pendulum Busch and A. flavum Hand.-Mazz., one of the most famous Tibetan medicines, is called Tiebangchui (TBC) in Chinese1,2. The dried roots of TBC are helpful in dispelling cold and wind, reducing pain, and calming shock. It was recorded in the first volume of "Drug Standards (Tibetan Medicine) of the Ministry of Health of the People's Republic of China," which states that the dried roots of TBC are commonly used to treat rheumatoid arthritis, bruises, and other cold diseases3. However, the clinical therapeutic dose of TBC is similar to its toxic dose, and incidents of poisoning or death have been frequently reported due to improper use4. Therefore, reducing the toxicity and preserving the efficacy of TBC has become a research hot spot over the years.
In Tibetan medicine, processing is one of the most effective methods to attenuate the toxicity of TBC. According to "Processing specification of Tibetan medicine of Qinghai Province (2010)", the original herbs (TBC) should be placed in an iron pot and stir-fried with Zanba until the Zanba turns yellow, after which Zanba is removed and the herbs are dried in air5,6. However, no specific process parameters have been documented, which makes controlling the processing technology and the quality of Zanba-stir-fried TBC difficult. The CRITIC method is an objective weight method that can avoid fuzzification and subjectivity, and enhance the objectivity of weighing7. The Box-Behnken response surface method can directly reflect the interaction between each factor through polynomial fitting8. The combination of the Box-Behnken response surface and CRITIC method is commonly used to optimize processing technology to acquire the optimized processing protocol9,10. In this paper, a monoester-diterpenoid alkaloid (MDA) (benzoylaconitine) and two diester-diterpenoid alkaloids (DDAs) (aconitine, 3-deoxyaconitine) were used as evaluation indexes. CRITIC combined with the Box-Behnken response surface method was applied to optimize the processing technology of Zanba-stir-fried TBC and establish a standard processing method for clinical safe use.

Autor im Rampenlicht: Optimierung der Verarbeitungstechnologie für Tiebangchui mit Zanba auf Basis von CRITIC in Kombination mit der Box-Behnken Response Surface Method  

Optimierung der Aufbereitungstechnik für Tiebangchui mit Zanba auf Basis von CRITIC in Kombination mit der Box-Behnken Response Surface Methode

The scope of this protocol lies in the proceeding of the Tibetan medicines. This staff aims to the optimize and standardize the processing technology of Zanba-stir-fried Tiebangchui. The combination of the Box-Behnken response surface and the CRITIC method is commonly used to optimize proceeding technology to acquire the optimized proceeding protocol.The optimized processing conditions of two centimeter Tiebangchui slice thickness three times more Zanba than Tiebangchui, a processing temperature of 125 degrees C and the 60 metres of stir-frying. By quantifying the specific processing parameters, this method will benefit the large-scale production and the quantity control of Zanba-stir-fried Tiebangchui. The standard Zanba-stir-fried processing technology of Tiebangchui can provide useful information and the guidance for the processing of other poisonous ethnical medicines.The optimized process is stable and feasible, laying a certain foundation for further investigation of Tiebangchui products and related Chinese medicine preparations. Our laboratory will pay more attention to cytochemical investigation to obtain the targeted quantity control compliance. In addition, the pharmacological comparison overall in Zanba-stir-fried Tiebangchui is unclear.Detoxification and evaluation of the efficacy, reservation effects in animal models will be the next objectives.

In dieser Studie hatte der verwendete Elutionsgradient eine gute Auflösung (Abbildung 1) für die drei Indexkomponenten in Zanba-gerührter TBC, wie nach wiederholtem Debuggen bestimmt. Die drei Indexkomponenten in Zanba-gerührtem TBC wiesen eine gute lineare Beziehung innerhalb eines bestimmten Konzentrationsbereichs auf (Tabelle 2). Die Präzision (Tabelle 5), die Stabilität (Tabelle 6), die Wiederholbarkeit (Tabelle 7) und die Probenrückgewinnung (<st...

Watch this Scientific Journal Video about Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method at JoVE.com

Das vorliegende Protokoll beschreibt eine effiziente und standardmäßige Entgiftungsverarbeitungsmethode für Zanba-Pfannengerichte Tiebangchui unter Verwendung von CRITIC in Kombination mit der Box-Behnken-Response-Surface-Methode.

The dried root of Aconitum pendulum Busch., called Tiebangchui (TBC) in Chinese, is one of the most famous Tibetan medicines. It is a widely used herb in ...

Der Geltungsbereich dieses Protokolls liegt im Verfahren der tibetischen Arzneimittel. Dieses Team hat sich zum Ziel gesetzt, die Verarbeitungstechnologie von Zanba-Pfannengerichten Tiebangchui zu optimieren und zu standardisieren. Die Kombination aus der Box-Behnken-Antwortfläche und der CRITIC-Methode wird häufig verwendet, um die Vorgehenstechnologie zu optimieren, um das optimierte Vorgehensprotokoll zu erhalten.Die optimierten Verarbeitungsbedingungen von zwei Zentimetern Tiebangchui-Scheibendicke dreimal mehr Zanba als Tiebangchui, eine Verarbeitungstemperatur von 125 Grad C und die 60 Meter Pfannengerichtsung. Durch die Quantifizierung der spezifischen Verarbeitungsparameter wird diese Methode der Großproduktion und der Mengenkontrolle von Zanba-Pfannengerichten Tiebangchui zugute kommen. Die standardmäßige Zanba-Pfannenrühr-Verarbeitungstechnologie von Tiebangchui kann nützliche Informationen und Anleitungen für die Verarbeitung anderer giftiger ethnischer Arzneimittel liefern.Der optimierte Prozess ist stabil und durchführbar und bildet eine gewisse Grundlage für die weitere Erforschung von Tiebangchui-Produkten und verwandten Präparaten für die chinesische Medizin. Unser Labor wird der zytochemischen Untersuchung mehr Aufmerksamkeit schenken, um die angestrebte Einhaltung der Mengenkontrolle zu erreichen. Darüber hinaus ist der pharmakologische Vergleich in Zanba-gerührtem Tiebangchui insgesamt unklar.Entgiftung und Evaluierung der Wirksamkeit, Vorbehaltseffekte im Tiermodell sind die nächsten Ziele.

optimization-processing-technology-for-tiebangchui-with-zanba-based

Research

JoVE Journal

Medicine

Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method

716 Aufrufe.  Chengdu University of Traditional Chinese Medicine. Der Geltungsbereich dieses Protokolls liegt im Verfahren der tibetischen Arzneimittel. Dieses Team hat sich zum Ziel gesetzt, die Verarbeitungstechnologie von Zanba-Pfannengerichten Tiebangchui zu optimieren und zu standardisieren. Die Kombination aus der Box-Behnken-Antwortfläche und der CRITIC-Methode wird häufig verwendet, um die Vorgehenstechnologie zu optimieren, um das optimierte Vorgehensprotokoll zu erhalten.Die optimierten Verarbeitungsbedingungen von zwei Zentimetern Tieban...

Serie: Autor im Rampenlicht: Optimierung der Verarbeitungstechnologie für Tiebangchui mit Zanba auf Basis von CRITIC in Kombination mit der Box-Behnken Response Surface Method  

The dried root of Aconitum pendulum Busch., called Tiebangchui (TBC) in Chinese, is one of the most famous Tibetan medicines. It is a widely used herb in northwest China. However, many cases of poisoning have occurred because of TBC's intense toxicity and because its therapeutic and toxic doses are similar. Therefore, finding a safe and effective method to reduce its toxicity is an urgent task. A search through the Tibetan medicine classics shows that the processing method of TBC stir-fried with Zanba was recorded in the "Processing specification of Tibetan medicine of Qinghai Province (2010)". However, the specific processing parameters are not yet clear. Thus, this study aims to optimize and standardize the processing technology of Zanba-stir-fried TBC.
First, a single-factor experiment was conducted on four factors: the slice thickness of TBC, amount of Zanba, processing temperature, and time. With monoester and diester alkaloid contents in Zanba-stir-fried TBC as indexes, CRITIC combined with the Box-Behnken response surface method was used to optimize the processing technology of Zanba-stir-fried TBC. The optimized processing conditions of Zanba-stir-fried TBC were a TBC slice thickness of 2 cm, three times more Zanba than TBC, a processing temperature of 125 &#176;C, and 60 min of stir-frying. This study determined the optimized and standard processing conditions for the usage of Zanba-stir-fried TBC, thus providing an experimental basis for the safe clinical use and industrial production of Zanba-stir-fried TBC.

The present protocol describes an efficient and standard detoxification processing method for Zanba-stir-fried Tiebangchui using CRITIC combined with the Box-Behnken response surface method.

Forschung

Medizin

Preparing Zanba-Stir-Fried Tiebangchui (TBC) Sample for Processing Technology Optimization

To begin a single factor experiment to compare Tiebangchui, or TBC slice thickness, for processing technology optimization, prepare five test groups each having 30 grams of TBC, but different slice thickness. Then weigh 90 grams of Zanba, which is three times as much as that of the TBC in each group. Set the temperature and the time of the automatic stir fry machine to 140 degrees Celsius and 40 minutes respectively.After the machine has heated up to the set temperature, add the weighed amounts of TBC and Zanba into the machine. Then using a high speed smashing machine, grind the Zanba stir-fried TBC into powder. Next, weigh two grams of Zanba stir-fried TBC powder in a conical flask.To the sample in the flask, add three milliliters of ammonia solution followed by 50 milliliters of a mixture of isopropyl alcohol and ethyl acetate, having a volume ratio of one is to one. After recording the weight of the conical flask with the contents, ultrasonicate the TBC sample for 30 minutes at 220 volts and 40 kilohertz maintaining a temperature below 25 degrees Celsius. Weigh the conical flask with the sample once again after the ultrasonic extraction.Make up for the lost weight by adding the isopropyl alcohol and ethyl acetate mixture mentioned previously, and filter the sample solution. Evaporate 25 milliliters of the filtrate to dryness using a rotary evaporator at 40 degrees Celsius. Then dissolve the residue in five milliliters of a solution of 0.05 hydrochloric acid in methanol.Filter the solution through a 0.2 micron syringe filter, and analyze it by performing high performance liquid chromatography, or HPLC. Calculate the contents of the MDA and DDAs in different processing products according to the standard curve. Calculate the comprehensive score based on the results via the critic method.Following the method demonstrated above, compare the amounts of Zanba, processing temperatures, and times for processing technology optimization. The results of the single factor tests revealed that the comprehensive scores of Zanba stir-fried TBC were highest when the sliced thickness is two centimeters, the amount of Zanba is three times, the processing temperature is 120 degrees Celsius, and the processing time is 60 minutes. The results revealed the range of slicing thickness, amount of Zanba, processing temperature, and processing time to be used for future experiments.

Vorbereitung der Zanba-Stir-Fried-Tiebangchui-Probe (TBC) für die Optimierung der Verarbeitungstechnologie

Response Surface Methodology (RSM) for Processing Technology Optimization of Zanba-Stir-Fried Tiebangchui (TBC)

To perform the Box-Behnken in response surface design for optimization of Zanba-Stir-Fried Tiebangchui or TBC processing technology. Use the range of sliced thickness the amount of Zanba, the processing temperature and the processing time determined by preliminary experiments using single factor tests. On the homepage.Click on new design, and in the left panel of the design page, click on response surface. Then click Box-Behnken and set the parameters for the four factors in the table. Click on next, set the response names and click on finish.To generate the response surface design. Complete the experiment based on the 29 scenarios designed for the response surface, using high performance liquid chromatography or HPLC. Record the peak areas and determine the quality of the MDA and DDAs in the Zanba Stir-fried TBC test samples.Then input the comprehensive scores obtained via the critic method for the 29 trials into the computer and analyze it using the referenced software, to perform the statistical validation of the polynomial equations in the left navigation pane. Under analysis plus, click on R1 and then click on start analysis. In the configure window, click on ANOVA in the top menu and observe the results table displaying variance analysis.Next in the top menu, click on model graphs and then 3D surface to obtain the response surface plots reflecting the effects of processing parameters on the synthetic scores. Go to the left navigation pane and under optimization click on numerical to validate the response surface model in triplicate under the predicted optimum conditions. Then in the top menu, click on solutions observe the predicted optimal conditions to verify the stability of the processing technology.The response surfaces and contour plots demonstrated the changes in synthetic scores as a function of four variables, on the basis of the experimental results. The adjusted optimal processing conditions were a slice thickness of two centimeters, three times more Zanba than TBC, a processing temperature of 125 degrees Celsius and a processing time of 60 minutes. The reliability of the model was proven through three tests that were conducted according to the obtained processing parameters.