이 연구는 중국 국립 자연 과학 재단 (No. 82130113), 중국 박사후 과학 재단 (No. 2021MD703800), 쓰촨성 과학 기술부 청소년 과학 재단 (No. 2022NSFSC1449) 및 청두 중국 전통 의학 대학의 "Xinglin Scholars"연구 진흥 프로그램 (No. BSH2021009).

Zanba-Stir-Fried Tiebangchui 공정 최적화 방법

<ol>
	<li>Li, C. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aconitum+pendulum+and+Aconitum+flavum:+A+narrative+review+on+traditional+uses,+phytochemistry,+bioactivities+and+processing+methods.">Aconitum pendulum and Aconitum flavum: A narrative review on traditional uses, phytochemistry, bioactivities and processing methods.</a> Journal of Ethnopharmacology. 292, 115216 (2022).</li><li>Wang, J., Meng, X. H., Chai, T., Yang, J. L., Shi, Y. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diterpenoid+alkaloids+and+one+lignan+from+the+roots+of+Aconitum+pendulum+Busch.">Diterpenoid alkaloids and one lignan from the roots of Aconitum pendulum Busch.</a> Natural Products and Bioprospecting. 9 (6), 419-423 (2019).</li><li>Yu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traditional+Tibetan+medicine:+therapeutic+potential+in+rheumatoid+arthritis.">Traditional Tibetan medicine: therapeutic potential in rheumatoid arthritis.</a> Frontiers In Pharmacology. 13, 938915 (2022).</li><li>Zhao, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=One+case+of+ventricular+arrhythmia+caused+by+poisoning+of+traditional+Chinese+medicine+Aconitum+pendulum+Busch.">One case of ventricular arrhythmia caused by poisoning of traditional Chinese medicine Aconitum pendulum Busch.</a> Journal of People's Military Medical. 61 (4), 346-348 (2018).</li><li>Qinghai Medical Products Administration. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Processing+specification+of+Tibetan+medicine+of+Qinghai+province.">Processing specification of Tibetan medicine of Qinghai province.</a> Qinghai Nationalities Publishing House. , 96-97 (2010).</li><li>Li, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+three+objective+weighting+methods+to+optimize+the+extraction+process+of+Jianwei+Chupi+granules.">Comparison of three objective weighting methods to optimize the extraction process of Jianwei Chupi granules.</a> Journal of Guangdong Pharmaceutical University. 38 (6), 91-97 (2022).</li><li>Feng, Z. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Processing+methods+and+the+underlying+detoxification+mechanisms+for+toxic+medicinal+materials+used+by+ethnic+minorities+in+China:+A+review.">Processing methods and the underlying detoxification mechanisms for toxic medicinal materials used by ethnic minorities in China: A review.</a> Journal of Ethnopharmacology. 305, 116126 (2023).</li><li>Hsu, Y. T., Su, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+Box-Behnken+design+to+investigate+the+effect+of+process+parameters+on+the+microparticle+production+of+ethenzamide+through+the+rapid+expansion+of+the+supercritical+solutions+process.">Application of Box-Behnken design to investigate the effect of process parameters on the microparticle production of ethenzamide through the rapid expansion of the supercritical solutions process.</a> Pharmaceutics. 12 (1), 42 (2020).</li><li>Cheng, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimization+of+the+baked+drying+technology+of+Cinnamomi+Ramulus+based+on+CRITIC+combined+with+box-behnken+response+surface+method.">Optimization of the baked drying technology of Cinnamomi Ramulus based on CRITIC combined with box-behnken response surface method.</a> Journal of Chinese Medicinal Materials. 2022 (8), 1838-1842 (2022).</li><li>Huang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimization+of+microwave+processing+technology+for+carbonized+Gardenia+jasminoides+by+Box-Behnken+response+surface+methodology+based+on+CRITIC+weighted+evaluation.">Optimization of microwave processing technology for carbonized Gardenia jasminoides by Box-Behnken response surface methodology based on CRITIC weighted evaluation.</a> Chinese Herbal Medicines. 48 (6), 1133-1138 (2017).</li><li>Elling, U., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Derivation+and+maintenance+of+mouse+haploid+embryonic+stem+cells.">Derivation and maintenance of mouse haploid embryonic stem cells.</a> Nature Protocols. 14 (7), 1991-2014 (2019).</li><li>Gu, J., Wang, Y. P., Ma, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+determinnation+of+three+diester+diterpenoid+alkaloids+in+the+toots+of+Aconiti+flavi+et+penduli+by+HPLC+method.">Simultaneous determinnation of three diester diterpenoid alkaloids in the toots of Aconiti flavi et penduli by HPLC method.</a> Chinese Pharmaceutical Affairs. 28 (6), 618-621 (2014).</li><li>Zhang, Y., Fu, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=UPLC+simultaneous+determination+of+six+esteric+alkaloids+components+in+Aconitum+Flaram+Hand.Mazz.">UPLC simultaneous determination of six esteric alkaloids components in Aconitum Flaram Hand.Mazz.</a> Asia-Pacific Traditional Medicine. 16 (5), 62-65 (2020).</li><li>Rumachik, N. G., Malaker, S. A., Paulk, N. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=VectorMOD:+Method+for+bottom-up+proteomic+characterization+of+rAAV+capsid+post-translational+modifications+and+vector+impurities.">VectorMOD: Method for bottom-up proteomic characterization of rAAV capsid post-translational modifications and vector impurities.</a> Frontiers In Immunology. 12, 657795 (2021).</li><li>Wang, Y. J., Tao, P., Wang, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Attenuated+structural+transformation+of+aconitine+during+sand+frying+process+and+antiarrhythmic+effect+of+its+converted+products.">Attenuated structural transformation of aconitine during sand frying process and antiarrhythmic effect of its converted products.</a> Evidence-Based Complementary and Alternative Medicine. 2021, 7243052 (2021).</li><li>Wang, H. P., Zhang, Y. B., Yang, X. W., Zhao, D. Q., Wang, Y. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+characterization+of+ginsenosides+in+the+roots+and+rhizomes+of+Panax+ginseng+by+UPLC-DAD-QTOF-MS/MS+and+simultaneous+determination+of+19+ginsenosides+by+HPLC-ESI-MS.">Rapid characterization of ginsenosides in the roots and rhizomes of Panax ginseng by UPLC-DAD-QTOF-MS/MS and simultaneous determination of 19 ginsenosides by HPLC-ESI-MS.</a> Journal of Ginseng Research. 40 (4), 382-394 (2016).</li><li>vander Leeuw, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pain+and+cognitive+function+among+older+adults+living+in+the+community.">Pain and cognitive function among older adults living in the community.</a> Journals of Gerontology Series A. Biological Sciences and Medical Sciences. 71 (3), 398-405 (2016).</li><li>Lao, D., Liu, R., Liang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+on+plasma+metabolomics+for+HIV/AIDS+patients+treated+by+HAART+based+on+LC/MS-MS.">Study on plasma metabolomics for HIV/AIDS patients treated by HAART based on LC/MS-MS.</a> Frontiers in Pharmacology. 13, 885386 (2022).</li><li>Li, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+the+effectiveness+of+VOC-contaminated+soil+preparation+based+on+AHP-CRITIC-TOPSIS+model.">Evaluation of the effectiveness of VOC-contaminated soil preparation based on AHP-CRITIC-TOPSIS model.</a> Chemosphere. 271, 129571 (2021).</li><li>Zhong, S., Chen, Y., Miao, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+improved+CRITIC+method+to+evaluate+thermal+coal+suppliers.">Using improved CRITIC method to evaluate thermal coal suppliers.</a> Scientific Reports. 13 (1), 195 (2023).</li><li>Lewis, N. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetically+levitated+mesenchymal+stem+cell+spheroids+cultured+with+a+collagen+gel+maintain+phenotype+and+quiescence.">Magnetically levitated mesenchymal stem cell spheroids cultured with a collagen gel maintain phenotype and quiescence.</a> Journal of Tissue Engineering. 8, (2017).</li><li>Chinese Pharmacopoeia Committee. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Pharmacopoeia of the People's Republic of China. 4, (2020).</li><li>Li, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+response+surface+methodology-optimized+ultrasound-assisted+pretreatment+extraction+on+the+composition+of+essential+oil+released+from+tribute+citrus+peels.">Effect of response surface methodology-optimized ultrasound-assisted pretreatment extraction on the composition of essential oil released from tribute citrus peels.</a> Frontiers in Nutrition. 9, 840780 (2022).</li><li>Liu, X. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hezi+inhibits+Tiebangchui-induced+cardiotoxicity+and+preserves+its+anti-rheumatoid+arthritis+effects+by+regulating+the+pharmacokinetics+of+aconitine+and+deoxyaconitine.">Hezi inhibits Tiebangchui-induced cardiotoxicity and preserves its anti-rheumatoid arthritis effects by regulating the pharmacokinetics of aconitine and deoxyaconitine.</a> Journal of Ethnopharmacology. 302, 115915 (2023).</li><li>Smolen, J. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rheumatoid+arthritis.">Rheumatoid arthritis.</a> Nature Reviews.Disease Primers. 4, 18001 (2018).</li><li>Wang, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=C19-norditerpenoid+alkaloids+from+Aconitum+szechenyianum+and+their+effects+on+LPS-activated+NO+production.">C19-norditerpenoid alkaloids from Aconitum szechenyianum and their effects on LPS-activated NO production.</a> Molecules. 21 (9), 1175 (2016).</li><li>Wang, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+on+the+alkaloids+in+Tibetan+medicine+Aconitum+pendulum+Busch+by+HPLC-MSn+combined+with+column+chromatography.">Study on the alkaloids in Tibetan medicine Aconitum pendulum Busch by HPLC-MSn combined with column chromatography.</a> Journal of Chromatographic Science. 54 (5), 752-758 (2016).</li><li>Liu, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+traditional+and+current+methods+used+to+potentially+reduce+toxicity+of+Aconitum+roots+in+Traditional+Chinese+Medicine.">A review of traditional and current methods used to potentially reduce toxicity of Aconitum roots in Traditional Chinese Medicine.</a> Journal of Ethnopharmacology. 207, 237-250 (2017).</li><li>Qiu, Z. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Online+discovery+of+the+molecular+mechanism+for+directionally+detoxification+of+Fuzi+using+real-time+extractive+electrospray+ionization+mass+spectrometry.">Online discovery of the molecular mechanism for directionally detoxification of Fuzi using real-time extractive electrospray ionization mass spectrometry.</a> Journal of Ethnopharmacology. 277, 114216 (2021).</li><li>El-Shazly, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use,+history,+and+liquid+chromatography/mass+spectrometry+chemical+analysis+of+Aconitum.">Use, history, and liquid chromatography/mass spectrometry chemical analysis of Aconitum.</a> Journal of Food and Drug Analysis. 24 (1), 29-45 (2016).</li><li>Chan, T. Y. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aconitum+alkaloid+poisoning+because+of+contamination+of+herbs+by+aconite+roots.">Aconitum alkaloid poisoning because of contamination of herbs by aconite roots.</a> Phytotherapy Research. 30 (1), 3-8 (2016).</li><li>Guo, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exploring+microbial+dynamics+associated+with+flavours+production+during+highland+barley+wine+fermentation.">Exploring microbial dynamics associated with flavours production during highland barley wine fermentation.</a> Food Research International. 130, 108971 (2020).</li><li>Guo, T. L., Horvath, C., Chen, L., Chen, J., Zheng, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Understanding+the+nutrient+composition+and+nutritional+functions+of+highland+barley+(Qingke):+A+review.">Understanding the nutrient composition and nutritional functions of highland barley (Qingke): A review.</a> Trends in Food Science &amp; Technology. 103, 109-117 (2020).</li><li>Wu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anti-myocardial+infarction+effects+of+Radix+Aconiti+Lateralis+Preparata+extracts+and+their+influence+on+small+molecules+in+the+heart+using+matrix-assisted+laser+desorption/ionization-mass+spectrometry+imaging.">Anti-myocardial infarction effects of Radix Aconiti Lateralis Preparata extracts and their influence on small molecules in the heart using matrix-assisted laser desorption/ionization-mass spectrometry imaging.</a> International Journal of Molecular Sciences. 20 (19), 4837 (2019).</li><li>Huang, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+on+cardiotoxicity+and+mechanism+of+&amp;#34;Fuzi&amp;#34;+extracts+based+on+metabonomics.">Study on cardiotoxicity and mechanism of &amp;#34;Fuzi&amp;#34; extracts based on metabonomics.</a> International Journal of Molecular Sciences. 19 (11), 3506 (2018).</li><li>Li, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+insight+into+current+advances+on+pharmacology,+pharmacokinetics,+toxicity+and+detoxification+of+aconitine.">An insight into current advances on pharmacology, pharmacokinetics, toxicity and detoxification of aconitine.</a> Biomedicine &amp; Pharmacotherapy. 151, 113115 (2022).</li><li>Xie, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimization+of+processing+technology+of+braised+Rehmanniae+Raidx+based+on+multiple+indexes+and+response+surface+technology+and+correlation+between+components+and+color.">Optimization of processing technology of braised Rehmanniae Raidx based on multiple indexes and response surface technology and correlation between components and color.</a> Journal of Chinese Traditional Medicine. 47 (18), 4927-4937 (2022).</li><li>Yang, X. Q., Xu, W., Xiao, C. P., Sun, J., Feng, Y. Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+on+processing+technology+of+Atractylodes+chinensis+with+rice+water+and+its+pharmacodynamics+of+anti-diarrhea.">Study on processing technology of Atractylodes chinensis with rice water and its pharmacodynamics of anti-diarrhea.</a> Chinese Herbal Medicines. 53 (1), 78-86 (2022).</li></ol>

저자는 공개할 이해 상충이 없습니다.

TBC는 감기를 쫓아내고 통증을 완화시키는 효과가 있는 중요한 티베트 약입니다. 그것은 주로 수천 년 동안 중국에서 외상성 부상과 류마티스 관절염을 치료하는 데 사용되었습니다24,25,26. 디테르페노이드 알칼로이드는 TBC27,28,29의 활성 및 독성 성분입니다...

가장 유명한 티베트 의약품 중 하나인 Aconitum pendulum Busch와 A. flavum Hand.-Mazz.의 말린 뿌리는 중국어 1,2로 Tiebangchui(TBC)라고 합니다. TBC의 말린 뿌리는 추위와 바람을 쫓아내고 통증을 줄이며 충격을 진정시키는 데 도움이 됩니다. TBC의 말린 뿌리가 류마티스 관절염, 타박상 및 기타 감기 질환 치료에 일반적으로 사용된다는 내용의 "중화 인민 공화국 보건부 의약품 기준 (티베트 의학)"의 첫 번째 권에 기록되어 있습니다3. 그러나 TBC의 임상적 치료 용량은 독성 용량과 유사하며, 부적절한 사용으로 인한 중독 또는 사망 사고가 빈번하게 보고되고 있다4. 따라서 TBC의 독성을 줄이고 효능을 보존하는 것은 수년에 걸쳐 연구 핫스팟이 되었습니다.
티베트 의학에서 가공은 TBC의 독성을 약화시키는 가장 효과적인 방법 중 하나입니다. "칭하이 성 티베트 의학의 가공 사양 (2010)"에 따르면, 원래 허브 (TBC)는 철 냄비에 넣고 잔바가 노랗게 변할 때까지 잔바와 함께 볶은 다음 잔바를 제거하고 허브를 공기 중에서 건조시켜야합니다 5,6. 그러나 특정 공정 매개변수가 문서화되지 않아 잔바 볶음 TBC의 가공 기술과 품질을 제어하기가 어렵습니다. CRITIC 방법은 퍼지화와 주관성을 피하고 계량7의 객관성을 향상시킬 수 있는 객관적인 중량 방법입니다. Box-Behnken 반응 곡면 방법은 다항식 피팅(8)을 통해 각 요인 간의 교호작용을 직접 반영할 수 있다. Box-Behnken 응답 표면과 CRITIC 방법의 조합은 최적화된 처리 프로토콜(9, 10)을 획득하기 위해 처리 기술을 최적화하는 데 일반적으로 사용됩니다. 본 논문에서는 모노에스테르-디테르페노이드 알칼로이드(MDA)(벤조일아코니틴)와 2개의 디에스테르-디테르페노이드 알칼로이드(DDA)(아코니틴, 3-데옥시아코니틴)를 평가 지표로 사용하였다. Box-Behnken 반응 표면 분석법과 결합된 CRITIC을 적용하여 잔바 볶음 TBC의 처리 기술을 최적화하고 임상적으로 안전한 사용을 위한 표준 처리 방법을 확립했습니다.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>3-Deoxyaconitine</td>
			<td>Chengdu Desite Biotechnology Co., Ltd.</td>
			<td>DST221109-033</td>
			<td></td>
		</tr>
		<tr>
			<td>Aconitine</td>
			<td>Chengdu Desite Biotechnology Co., Ltd.</td>
			<td>DSTDW000602</td>
			<td></td>
		</tr>
		<tr>
			<td>Ammonium acetate</td>
			<td>Tianjin Kermel Chemical Reagent Co., Ltd</td>
			<td>Chromatographic grade</td>
			<td></td>
		</tr>
		<tr>
			<td>Benzoylaconitine</td>
			<td>Chengdu Desite Biotechnology Co., Ltd.</td>
			<td>DSTDB005502</td>
			<td></td>
		</tr>
		<tr>
			<td>Design-Expert software</td>
			<td>Stat-Ease, Inc., Minneapolis, MN, USA</td>
			<td>version 13.0</td>
			<td></td>
		</tr>
		<tr>
			<td>Electronic analytical balance</td>
			<td>Shanghai Liangping Instruments Co., Ltd.</td>
			<td>FA1004</td>
			<td></td>
		</tr>
		<tr>
			<td>High performance liquid chromatography</td>
			<td>SHIMADZU Co., Ltd.</td>
			<td>LC-20A</td>
			<td></td>
		</tr>
		<tr>
			<td>High-speed smashing machine</td>
			<td>Beijing Zhongxing Weiye Instrument Co., Ltd.</td>
			<td>FW-100</td>
			<td></td>
		</tr>
		<tr>
			<td>Millipore filter</td>
			<td>Tianjin Jinteng Experimental Equipment Co., Ltd</td>
			<td>&#966;13 0.22 Nylon66</td>
			<td></td>
		</tr>
		<tr>
			<td>stir-Fry machine</td>
			<td>Changzhou Maisi Machinery Co., Ltd</td>
			<td>Type 5</td>
			<td></td>
		</tr>
		<tr>
			<td>Tiebangchui</td>
			<td>Gannan Baicao Biotechnology Development Co., Ltd</td>
			<td>20211012</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultra pure water systemic</td>
			<td>RephiLe Bioscience, Ltd.</td>
			<td>Genie G</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultrasonic cleansing machine</td>
			<td>Ningbo Xinyi Ultrasonic Equipment Co., Ltd</td>
			<td>SB2200</td>
			<td></td>
		</tr>
		<tr>
			<td>Zanba</td>
			<td>27 Chuanzang Road, Ganzi County</td>
			<td>-</td>
			<td></td>
		</tr>
	</tbody>
</table>

optimization of processing technology for tiebangchui with zanba based on critic combined with box-behnken response surface method

중국어로 Tiebangchui(TBC)라고 불리는 Aconitum pendulum Busch.의 말린 뿌리는 가장 유명한 티베트 의약품 중 하나입니다. 중국 북서부에서 널리 사용되는 허브입니다. 그러나 TBC의 강렬한 독성과 치료 및 독성 용량이 유사하기 때문에 많은 중독 사례가 발생했습니다. 따라서 독성을 줄이기위한 안전하고 효과적인 방법을 찾는 것이 시급한 과제입니다. 티베트 의학 고전을 검색하면 잔바 볶음 TBC의 가공 방법이 "칭하이 성 티베트 의학 가공 사양 (2010)"에 기록되어 있음을 알 수 있습니다. 그러나 특정 처리 매개 변수는 아직 명확하지 않습니다. 따라서 본 연구는 잔바 볶음 TBC의 가공 기술을 최적화하고 표준화하는 것을 목표로 한다.
먼저, TBC의 슬라이스 두께, 잔바의 양, 가공 온도 및 시간의 4가지 요인에 대해 단일 요인 실험을 수행했습니다. 잔바 볶음 TBC의 모노에스테르 및 디에스테르 알칼로이드 함량을 지표로 하여 Box-Behnken 반응 표면 방법과 결합된 CRITIC을 사용하여 잔바 볶음 TBC의 가공 기술을 최적화했습니다. 잔바 볶음 TBC의 최적화된 가공 조건은 TBC 슬라이스 두께 2cm, TBC보다 3배 더 많은 잔바, 처리 온도 125°C, 볶음 60분이었다. 본 연구는 잔바 볶음 TBC의 사용을 위한 최적화된 표준 처리 조건을 결정하여 잔바 볶음 TBC의 안전한 임상 사용 및 산업 생산을 위한 실험 기반을 제공했습니다.

The dried root of Aconitum pendulum Busch and A. flavum Hand.-Mazz., one of the most famous Tibetan medicines, is called Tiebangchui (TBC) in Chinese1,2. The dried roots of TBC are helpful in dispelling cold and wind, reducing pain, and calming shock. It was recorded in the first volume of "Drug Standards (Tibetan Medicine) of the Ministry of Health of the People's Republic of China," which states that the dried roots of TBC are commonly used to treat rheumatoid arthritis, bruises, and other cold diseases3. However, the clinical therapeutic dose of TBC is similar to its toxic dose, and incidents of poisoning or death have been frequently reported due to improper use4. Therefore, reducing the toxicity and preserving the efficacy of TBC has become a research hot spot over the years.
In Tibetan medicine, processing is one of the most effective methods to attenuate the toxicity of TBC. According to "Processing specification of Tibetan medicine of Qinghai Province (2010)", the original herbs (TBC) should be placed in an iron pot and stir-fried with Zanba until the Zanba turns yellow, after which Zanba is removed and the herbs are dried in air5,6. However, no specific process parameters have been documented, which makes controlling the processing technology and the quality of Zanba-stir-fried TBC difficult. The CRITIC method is an objective weight method that can avoid fuzzification and subjectivity, and enhance the objectivity of weighing7. The Box-Behnken response surface method can directly reflect the interaction between each factor through polynomial fitting8. The combination of the Box-Behnken response surface and CRITIC method is commonly used to optimize processing technology to acquire the optimized processing protocol9,10. In this paper, a monoester-diterpenoid alkaloid (MDA) (benzoylaconitine) and two diester-diterpenoid alkaloids (DDAs) (aconitine, 3-deoxyaconitine) were used as evaluation indexes. CRITIC combined with the Box-Behnken response surface method was applied to optimize the processing technology of Zanba-stir-fried TBC and establish a standard processing method for clinical safe use.

저자 스포트라이트: Box-Behnken 응답 표면 방법과 결합된 CRITIC 기반 Zanba를 사용한 Tiebangchui의 처리 기술 최적화  

Box-Behnken Response Surface Method와 결합된 CRITIC 기반 Tiebangchui with Zanba의 처리 기술 최적화

The scope of this protocol lies in the proceeding of the Tibetan medicines. This staff aims to the optimize and standardize the processing technology of Zanba-stir-fried Tiebangchui. The combination of the Box-Behnken response surface and the CRITIC method is commonly used to optimize proceeding technology to acquire the optimized proceeding protocol.The optimized processing conditions of two centimeter Tiebangchui slice thickness three times more Zanba than Tiebangchui, a processing temperature of 125 degrees C and the 60 metres of stir-frying. By quantifying the specific processing parameters, this method will benefit the large-scale production and the quantity control of Zanba-stir-fried Tiebangchui. The standard Zanba-stir-fried processing technology of Tiebangchui can provide useful information and the guidance for the processing of other poisonous ethnical medicines.The optimized process is stable and feasible, laying a certain foundation for further investigation of Tiebangchui products and related Chinese medicine preparations. Our laboratory will pay more attention to cytochemical investigation to obtain the targeted quantity control compliance. In addition, the pharmacological comparison overall in Zanba-stir-fried Tiebangchui is unclear.Detoxification and evaluation of the efficacy, reservation effects in animal models will be the next objectives.

이 연구에서 사용된 용출 그래디언트는 반복 디버깅 후 결정된 바와 같이 잔바 볶음 TBC의 세 가지 인덱스 성분에 대해 우수한 분해능을 보였습니다(그림 1). 잔바 볶음 TBC의 세 가지 지표 성분은 특정 농도 범위 내에서 양호한 선형 관계를 가졌다(표 2). 잔바 볶음 TBC의 정밀도(표 5), 안정성(표 6), 반복성(표 7) 및 시료 회수율(표 8</str...

Watch this Scientific Journal Video about Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method at JoVE.com

본 프로토콜은 Box-Behnken 반응 표면 방법과 결합된 CRITIC을 사용하여 잔바 볶음 Tiebangchui에 대한 효율적이고 표준적인 해독 처리 방법을 설명합니다.

The dried root of Aconitum pendulum Busch., called Tiebangchui (TBC) in Chinese, is one of the most famous Tibetan medicines. It is a widely used herb in ...

이 프로토콜의 범위는 티베트 의약품의 진행에 있습니다. 이 직원은 잔바 볶음 Tiebangchui의 가공 기술을 최적화하고 표준화하는 것을 목표로합니다. Box-Behnken 응답 표면과 CRITIC 방법의 조합은 일반적으로 최적화된 진행 프로토콜을 획득하기 위해 진행 기술을 최적화하는 데 사용됩니다.Tiebangchui보다 3배 더 많은 잔바 두께의 2cm Tiebangchui 슬라이스의 최적화된 가공 조건, 125°C의 가공 온도 및 60m의 볶음 요리. 특정 처리 매개 변수를 정량화함으로써이 방법은 Zanba-stir-fried Tiebangchui의 대규모 생산 및 수량 관리에 도움이 될 것입니다. Tiebangchui의 표준 잔바 볶음 가공 기술은 다른 유독 한 민족 의약품 가공에 유용한 정보와 지침을 제공 할 수 있습니다.최적화된 공정은 안정적이고 실현 가능하며 Tiebangchui 제품 및 관련 한약 제제에 대한 추가 조사를 위한 특정 토대를 마련합니다. 우리 실험실은 목표 수량 제어 준수를 얻기 위해 세포 화학적 조사에 더 많은 관심을 기울일 것입니다. 또한, 잔바 볶음 Tiebangchui의 전반적인 약리학적 비교는 불분명하다.동물모델에서 해독 및 효능, 예약 효과의 평가가 다음 목표가 될 것이다.

optimization-processing-technology-for-tiebangchui-with-zanba-based

Research

JoVE Journal

Medicine

Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method

715 조회수.  Chengdu University of Traditional Chinese Medicine. 이 프로토콜의 범위는 티베트 의약품의 진행에 있습니다. 이 직원은 잔바 볶음 Tiebangchui의 가공 기술을 최적화하고 표준화하는 것을 목표로합니다. Box-Behnken 응답 표면과 CRITIC 방법의 조합은 일반적으로 최적화된 진행 프로토콜을 획득하기 위해 진행 기술을 최적화하는 데 사용됩니다.Tiebangchui보다 3배 더 많은 잔바 두께의 2cm Tiebangchui 슬라이스의 최적화된 가공 조건, 12...

비디오: 저자 스포트라이트: Box-Behnken 응답 표면 방법과 결합된 CRITIC 기반 Zanba를 사용한 Tiebangchui의 처리 기술 최적화  

The dried root of Aconitum pendulum Busch., called Tiebangchui (TBC) in Chinese, is one of the most famous Tibetan medicines. It is a widely used herb in northwest China. However, many cases of poisoning have occurred because of TBC's intense toxicity and because its therapeutic and toxic doses are similar. Therefore, finding a safe and effective method to reduce its toxicity is an urgent task. A search through the Tibetan medicine classics shows that the processing method of TBC stir-fried with Zanba was recorded in the "Processing specification of Tibetan medicine of Qinghai Province (2010)". However, the specific processing parameters are not yet clear. Thus, this study aims to optimize and standardize the processing technology of Zanba-stir-fried TBC.
First, a single-factor experiment was conducted on four factors: the slice thickness of TBC, amount of Zanba, processing temperature, and time. With monoester and diester alkaloid contents in Zanba-stir-fried TBC as indexes, CRITIC combined with the Box-Behnken response surface method was used to optimize the processing technology of Zanba-stir-fried TBC. The optimized processing conditions of Zanba-stir-fried TBC were a TBC slice thickness of 2 cm, three times more Zanba than TBC, a processing temperature of 125 &#176;C, and 60 min of stir-frying. This study determined the optimized and standard processing conditions for the usage of Zanba-stir-fried TBC, thus providing an experimental basis for the safe clinical use and industrial production of Zanba-stir-fried TBC.

The present protocol describes an efficient and standard detoxification processing method for Zanba-stir-fried Tiebangchui using CRITIC combined with the Box-Behnken response surface method.

연구

의학

Preparing Zanba-Stir-Fried Tiebangchui (TBC) Sample for Processing Technology Optimization

To begin a single factor experiment to compare Tiebangchui, or TBC slice thickness, for processing technology optimization, prepare five test groups each having 30 grams of TBC, but different slice thickness. Then weigh 90 grams of Zanba, which is three times as much as that of the TBC in each group. Set the temperature and the time of the automatic stir fry machine to 140 degrees Celsius and 40 minutes respectively.After the machine has heated up to the set temperature, add the weighed amounts of TBC and Zanba into the machine. Then using a high speed smashing machine, grind the Zanba stir-fried TBC into powder. Next, weigh two grams of Zanba stir-fried TBC powder in a conical flask.To the sample in the flask, add three milliliters of ammonia solution followed by 50 milliliters of a mixture of isopropyl alcohol and ethyl acetate, having a volume ratio of one is to one. After recording the weight of the conical flask with the contents, ultrasonicate the TBC sample for 30 minutes at 220 volts and 40 kilohertz maintaining a temperature below 25 degrees Celsius. Weigh the conical flask with the sample once again after the ultrasonic extraction.Make up for the lost weight by adding the isopropyl alcohol and ethyl acetate mixture mentioned previously, and filter the sample solution. Evaporate 25 milliliters of the filtrate to dryness using a rotary evaporator at 40 degrees Celsius. Then dissolve the residue in five milliliters of a solution of 0.05 hydrochloric acid in methanol.Filter the solution through a 0.2 micron syringe filter, and analyze it by performing high performance liquid chromatography, or HPLC. Calculate the contents of the MDA and DDAs in different processing products according to the standard curve. Calculate the comprehensive score based on the results via the critic method.Following the method demonstrated above, compare the amounts of Zanba, processing temperatures, and times for processing technology optimization. The results of the single factor tests revealed that the comprehensive scores of Zanba stir-fried TBC were highest when the sliced thickness is two centimeters, the amount of Zanba is three times, the processing temperature is 120 degrees Celsius, and the processing time is 60 minutes. The results revealed the range of slicing thickness, amount of Zanba, processing temperature, and processing time to be used for future experiments.

가공 기술 최적화를 위한 잔바 볶음 Tiebangchui (TBC) 샘플 준비

Response Surface Methodology (RSM) for Processing Technology Optimization of Zanba-Stir-Fried Tiebangchui (TBC)

To perform the Box-Behnken in response surface design for optimization of Zanba-Stir-Fried Tiebangchui or TBC processing technology. Use the range of sliced thickness the amount of Zanba, the processing temperature and the processing time determined by preliminary experiments using single factor tests. On the homepage.Click on new design, and in the left panel of the design page, click on response surface. Then click Box-Behnken and set the parameters for the four factors in the table. Click on next, set the response names and click on finish.To generate the response surface design. Complete the experiment based on the 29 scenarios designed for the response surface, using high performance liquid chromatography or HPLC. Record the peak areas and determine the quality of the MDA and DDAs in the Zanba Stir-fried TBC test samples.Then input the comprehensive scores obtained via the critic method for the 29 trials into the computer and analyze it using the referenced software, to perform the statistical validation of the polynomial equations in the left navigation pane. Under analysis plus, click on R1 and then click on start analysis. In the configure window, click on ANOVA in the top menu and observe the results table displaying variance analysis.Next in the top menu, click on model graphs and then 3D surface to obtain the response surface plots reflecting the effects of processing parameters on the synthetic scores. Go to the left navigation pane and under optimization click on numerical to validate the response surface model in triplicate under the predicted optimum conditions. Then in the top menu, click on solutions observe the predicted optimal conditions to verify the stability of the processing technology.The response surfaces and contour plots demonstrated the changes in synthetic scores as a function of four variables, on the basis of the experimental results. The adjusted optimal processing conditions were a slice thickness of two centimeters, three times more Zanba than TBC, a processing temperature of 125 degrees Celsius and a processing time of 60 minutes. The reliability of the model was proven through three tests that were conducted according to the obtained processing parameters.