Ethyl Acetate Hydrocarbon Extraction and Concentration Measurements
7:40
Alcohol/Aldehyde Dehydrogenase Activity Assay
8:49
pCaiF Characterization and Tolerance Assay
10:26
Homolog Interaction Mapping
11:11
Representative Hydrocarbon Conversion Results
19:46
Conclusion
A sustainable auto regulating bacterial system for the remediation of oil pollutions was designed using standard interchangeable DNA parts (BioBricks). An engineered E. coli strain was used to degrade alkanes via β-oxidation in toxic aqueous environments. The respective enzymes from different species showed alkane degradation activity. Additionally, an increased tolerance to n-hexane was achieved by introducing genes from alkane-tolerant bacteria.