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JoVE Journal

Biochemistry

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An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

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07:46 min

October 8th, 2018

October 8th, 2018

6,821 Views

0:04

Title

0:39

Preparing a 20% Polyacrylamide Denaturing Gel

2:17

RNA Isopentenylation Assay

5:14

Results: Detection of Isopentenylation by Mod5

6:42

Conclusion

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The overall goal of this protocol is to biochemically characterize tRNA-Isopentenyl Transferase Activity In Vitro. This method is useful for the in-vitro detection and characterization of tRNA-isopentenyl transferase activity of purified recombina

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Here, we describe a protocol for the biochemical characterization of the yeast RNA-modifying enzyme, Mod5, and discuss how this protocol could be applied to other RNA-modifying enzymes.

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