For this method can help answer key question in the field of inflammation and immunology. For example, what are the molecular mechanism of leukocyte adhesion and trans-endothelial migration. The main advantage of this technique is that it allows u
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Here, we present an integrated protocol that measures monocyte subpopulation trafficking under flow in vitro by use of specific surface markers and confocal fluorescence microscopy. This protocol can be used to explore sequential recruitment steps as well as to profile other leukocyte subtypes using other specific surface markers.