Characterization and Banking of Established Primary Tumor Cell Line
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Results: Determination of Fibroblast Contamination of Cell Culture and Flow Cytometry Surface Staining of Cell Lines
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Conclusion
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This protocol provides a basis for tumor cell expansion that, in turn, allows for an in-depth study of tumor cell function and genomic states. In addition, it provides an in vitro model system to assess the impact of the tumor on the immune respon
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The goal of this method paper is to demonstrate a robust and reproducible methodology for the enrichment, generation, and expansion of primary tumor cell lines from surgically resected pleural mesothelioma.