Divergent Primer Design for circRNA and RNA Isolation
3:16
cDNA Synthesis
4:09
Digital Droplet PCR (dd-PCR) Workflow
7:04
Results: Data Analysis Using QuantaSoft Software
8:05
Conclusion
Transcripción
dd-PCR is a highly sensitive and accurate method for absolute quantification of target DNA or RNA. It has been used for disease diagnosis where other conventional methods fail to give accurate results. dd-PCR is the most sensitive technique for de
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This protocol describes the detailed method of digital droplet PCR (dd-PCR) for precise quantification of circular RNA (circRNA) levels in cells using divergent primers.