September 16th, 2022
•This protocol describes the detailed method of digital droplet PCR (dd-PCR) for precise quantification of circular RNA (circRNA) levels in cells using divergent primers.
Related Videos
Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit
Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
Rapid PCR Thermocycling using Microscale Thermal Convection
Isolation of Small Noncoding RNAs from Human Serum
Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR
Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
Copyright © 2024 MyJoVE Corporation. 판권 소유