Name: Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method (Video) | JoVE
Uploaded: 2023-05-12T13:20:00
Description: Watch this Scientific Journal Video about Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method at JoVE.com

This work was financially supported by the National Natural Science Foundation of China (No. 82130113), the China Postdoctoral Science Foundation (No. 2021MD703800), the Science Foundation for Youths of Science &#38; Technology Department of Sichuan Province (No. 2022NSFSC1449), and the &#34;Xinglin Scholars&#34; Research Promotion Program of Chengdu University of Traditional Chinese Medicine (No. BSH2021009).

The Zanba-stir-fried TBC processing method was optimized and standardized by CRITIC combined with the Box-Behnken response surface method. Benzoylaconitine, aconitine, and 3-deoxyaconitine were used as evaluation indexes during this procedure.
1. Sample solution preparation
<ol>
	<li>Prepare the reference substance stock solution. Weigh precisely 9.94 mg of benzoylaconitine, 8.49 mg of aconitine, and 6.25 mg of 3-deoxyaconitine (Table of Materials) on an ele...

A Method for Zanba-Stir-Fried Tiebangchui Process Optimization

<ol>
	<li>Li, C. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aconitum+pendulum+and+Aconitum+flavum:+A+narrative+review+on+traditional+uses,+phytochemistry,+bioactivities+and+processing+methods.">Aconitum pendulum and Aconitum flavum: A narrative review on traditional uses, phytochemistry, bioactivities and processing methods.</a> Journal of Ethnopharmacology. 292, 115216 (2022).</li><li>Wang, J., Meng, X. H., Chai, T., Yang, J. L., Shi, Y. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diterpenoid+alkaloids+and+one+lignan+from+the+roots+of+Aconitum+pendulum+Busch.">Diterpenoid alkaloids and one lignan from the roots of Aconitum pendulum Busch.</a> Natural Products and Bioprospecting. 9 (6), 419-423 (2019).</li><li>Yu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traditional+Tibetan+medicine:+therapeutic+potential+in+rheumatoid+arthritis.">Traditional Tibetan medicine: therapeutic potential in rheumatoid arthritis.</a> Frontiers In Pharmacology. 13, 938915 (2022).</li><li>Zhao, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=One+case+of+ventricular+arrhythmia+caused+by+poisoning+of+traditional+Chinese+medicine+Aconitum+pendulum+Busch.">One case of ventricular arrhythmia caused by poisoning of traditional Chinese medicine Aconitum pendulum Busch.</a> Journal of People's Military Medical. 61 (4), 346-348 (2018).</li><li>Qinghai Medical Products Administration. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Processing+specification+of+Tibetan+medicine+of+Qinghai+province.">Processing specification of Tibetan medicine of Qinghai province.</a> Qinghai Nationalities Publishing House. , 96-97 (2010).</li><li>Li, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+three+objective+weighting+methods+to+optimize+the+extraction+process+of+Jianwei+Chupi+granules.">Comparison of three objective weighting methods to optimize the extraction process of Jianwei Chupi granules.</a> Journal of Guangdong Pharmaceutical University. 38 (6), 91-97 (2022).</li><li>Feng, Z. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Processing+methods+and+the+underlying+detoxification+mechanisms+for+toxic+medicinal+materials+used+by+ethnic+minorities+in+China:+A+review.">Processing methods and the underlying detoxification mechanisms for toxic medicinal materials used by ethnic minorities in China: A review.</a> Journal of Ethnopharmacology. 305, 116126 (2023).</li><li>Hsu, Y. T., Su, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+Box-Behnken+design+to+investigate+the+effect+of+process+parameters+on+the+microparticle+production+of+ethenzamide+through+the+rapid+expansion+of+the+supercritical+solutions+process.">Application of Box-Behnken design to investigate the effect of process parameters on the microparticle production of ethenzamide through the rapid expansion of the supercritical solutions process.</a> Pharmaceutics. 12 (1), 42 (2020).</li><li>Cheng, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimization+of+the+baked+drying+technology+of+Cinnamomi+Ramulus+based+on+CRITIC+combined+with+box-behnken+response+surface+method.">Optimization of the baked drying technology of Cinnamomi Ramulus based on CRITIC combined with box-behnken response surface method.</a> Journal of Chinese Medicinal Materials. 2022 (8), 1838-1842 (2022).</li><li>Huang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimization+of+microwave+processing+technology+for+carbonized+Gardenia+jasminoides+by+Box-Behnken+response+surface+methodology+based+on+CRITIC+weighted+evaluation.">Optimization of microwave processing technology for carbonized Gardenia jasminoides by Box-Behnken response surface methodology based on CRITIC weighted evaluation.</a> Chinese Herbal Medicines. 48 (6), 1133-1138 (2017).</li><li>Elling, U., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Derivation+and+maintenance+of+mouse+haploid+embryonic+stem+cells.">Derivation and maintenance of mouse haploid embryonic stem cells.</a> Nature Protocols. 14 (7), 1991-2014 (2019).</li><li>Gu, J., Wang, Y. P., Ma, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+determinnation+of+three+diester+diterpenoid+alkaloids+in+the+toots+of+Aconiti+flavi+et+penduli+by+HPLC+method.">Simultaneous determinnation of three diester diterpenoid alkaloids in the toots of Aconiti flavi et penduli by HPLC method.</a> Chinese Pharmaceutical Affairs. 28 (6), 618-621 (2014).</li><li>Zhang, Y., Fu, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=UPLC+simultaneous+determination+of+six+esteric+alkaloids+components+in+Aconitum+Flaram+Hand.Mazz.">UPLC simultaneous determination of six esteric alkaloids components in Aconitum Flaram Hand.Mazz.</a> Asia-Pacific Traditional Medicine. 16 (5), 62-65 (2020).</li><li>Rumachik, N. G., Malaker, S. A., Paulk, N. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=VectorMOD:+Method+for+bottom-up+proteomic+characterization+of+rAAV+capsid+post-translational+modifications+and+vector+impurities.">VectorMOD: Method for bottom-up proteomic characterization of rAAV capsid post-translational modifications and vector impurities.</a> Frontiers In Immunology. 12, 657795 (2021).</li><li>Wang, Y. J., Tao, P., Wang, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Attenuated+structural+transformation+of+aconitine+during+sand+frying+process+and+antiarrhythmic+effect+of+its+converted+products.">Attenuated structural transformation of aconitine during sand frying process and antiarrhythmic effect of its converted products.</a> Evidence-Based Complementary and Alternative Medicine. 2021, 7243052 (2021).</li><li>Wang, H. P., Zhang, Y. B., Yang, X. W., Zhao, D. Q., Wang, Y. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+characterization+of+ginsenosides+in+the+roots+and+rhizomes+of+Panax+ginseng+by+UPLC-DAD-QTOF-MS/MS+and+simultaneous+determination+of+19+ginsenosides+by+HPLC-ESI-MS.">Rapid characterization of ginsenosides in the roots and rhizomes of Panax ginseng by UPLC-DAD-QTOF-MS/MS and simultaneous determination of 19 ginsenosides by HPLC-ESI-MS.</a> Journal of Ginseng Research. 40 (4), 382-394 (2016).</li><li>vander Leeuw, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pain+and+cognitive+function+among+older+adults+living+in+the+community.">Pain and cognitive function among older adults living in the community.</a> Journals of Gerontology Series A. Biological Sciences and Medical Sciences. 71 (3), 398-405 (2016).</li><li>Lao, D., Liu, R., Liang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+on+plasma+metabolomics+for+HIV/AIDS+patients+treated+by+HAART+based+on+LC/MS-MS.">Study on plasma metabolomics for HIV/AIDS patients treated by HAART based on LC/MS-MS.</a> Frontiers in Pharmacology. 13, 885386 (2022).</li><li>Li, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+the+effectiveness+of+VOC-contaminated+soil+preparation+based+on+AHP-CRITIC-TOPSIS+model.">Evaluation of the effectiveness of VOC-contaminated soil preparation based on AHP-CRITIC-TOPSIS model.</a> Chemosphere. 271, 129571 (2021).</li><li>Zhong, S., Chen, Y., Miao, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+improved+CRITIC+method+to+evaluate+thermal+coal+suppliers.">Using improved CRITIC method to evaluate thermal coal suppliers.</a> Scientific Reports. 13 (1), 195 (2023).</li><li>Lewis, N. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetically+levitated+mesenchymal+stem+cell+spheroids+cultured+with+a+collagen+gel+maintain+phenotype+and+quiescence.">Magnetically levitated mesenchymal stem cell spheroids cultured with a collagen gel maintain phenotype and quiescence.</a> Journal of Tissue Engineering. 8, (2017).</li><li>Chinese Pharmacopoeia Committee. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Pharmacopoeia of the People's Republic of China. 4, (2020).</li><li>Li, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+response+surface+methodology-optimized+ultrasound-assisted+pretreatment+extraction+on+the+composition+of+essential+oil+released+from+tribute+citrus+peels.">Effect of response surface methodology-optimized ultrasound-assisted pretreatment extraction on the composition of essential oil released from tribute citrus peels.</a> Frontiers in Nutrition. 9, 840780 (2022).</li><li>Liu, X. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hezi+inhibits+Tiebangchui-induced+cardiotoxicity+and+preserves+its+anti-rheumatoid+arthritis+effects+by+regulating+the+pharmacokinetics+of+aconitine+and+deoxyaconitine.">Hezi inhibits Tiebangchui-induced cardiotoxicity and preserves its anti-rheumatoid arthritis effects by regulating the pharmacokinetics of aconitine and deoxyaconitine.</a> Journal of Ethnopharmacology. 302, 115915 (2023).</li><li>Smolen, J. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rheumatoid+arthritis.">Rheumatoid arthritis.</a> Nature Reviews.Disease Primers. 4, 18001 (2018).</li><li>Wang, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=C19-norditerpenoid+alkaloids+from+Aconitum+szechenyianum+and+their+effects+on+LPS-activated+NO+production.">C19-norditerpenoid alkaloids from Aconitum szechenyianum and their effects on LPS-activated NO production.</a> Molecules. 21 (9), 1175 (2016).</li><li>Wang, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+on+the+alkaloids+in+Tibetan+medicine+Aconitum+pendulum+Busch+by+HPLC-MSn+combined+with+column+chromatography.">Study on the alkaloids in Tibetan medicine Aconitum pendulum Busch by HPLC-MSn combined with column chromatography.</a> Journal of Chromatographic Science. 54 (5), 752-758 (2016).</li><li>Liu, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+traditional+and+current+methods+used+to+potentially+reduce+toxicity+of+Aconitum+roots+in+Traditional+Chinese+Medicine.">A review of traditional and current methods used to potentially reduce toxicity of Aconitum roots in Traditional Chinese Medicine.</a> Journal of Ethnopharmacology. 207, 237-250 (2017).</li><li>Qiu, Z. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Online+discovery+of+the+molecular+mechanism+for+directionally+detoxification+of+Fuzi+using+real-time+extractive+electrospray+ionization+mass+spectrometry.">Online discovery of the molecular mechanism for directionally detoxification of Fuzi using real-time extractive electrospray ionization mass spectrometry.</a> Journal of Ethnopharmacology. 277, 114216 (2021).</li><li>El-Shazly, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use,+history,+and+liquid+chromatography/mass+spectrometry+chemical+analysis+of+Aconitum.">Use, history, and liquid chromatography/mass spectrometry chemical analysis of Aconitum.</a> Journal of Food and Drug Analysis. 24 (1), 29-45 (2016).</li><li>Chan, T. Y. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aconitum+alkaloid+poisoning+because+of+contamination+of+herbs+by+aconite+roots.">Aconitum alkaloid poisoning because of contamination of herbs by aconite roots.</a> Phytotherapy Research. 30 (1), 3-8 (2016).</li><li>Guo, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exploring+microbial+dynamics+associated+with+flavours+production+during+highland+barley+wine+fermentation.">Exploring microbial dynamics associated with flavours production during highland barley wine fermentation.</a> Food Research International. 130, 108971 (2020).</li><li>Guo, T. L., Horvath, C., Chen, L., Chen, J., Zheng, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Understanding+the+nutrient+composition+and+nutritional+functions+of+highland+barley+(Qingke):+A+review.">Understanding the nutrient composition and nutritional functions of highland barley (Qingke): A review.</a> Trends in Food Science &amp; Technology. 103, 109-117 (2020).</li><li>Wu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anti-myocardial+infarction+effects+of+Radix+Aconiti+Lateralis+Preparata+extracts+and+their+influence+on+small+molecules+in+the+heart+using+matrix-assisted+laser+desorption/ionization-mass+spectrometry+imaging.">Anti-myocardial infarction effects of Radix Aconiti Lateralis Preparata extracts and their influence on small molecules in the heart using matrix-assisted laser desorption/ionization-mass spectrometry imaging.</a> International Journal of Molecular Sciences. 20 (19), 4837 (2019).</li><li>Huang, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+on+cardiotoxicity+and+mechanism+of+&amp;#34;Fuzi&amp;#34;+extracts+based+on+metabonomics.">Study on cardiotoxicity and mechanism of &amp;#34;Fuzi&amp;#34; extracts based on metabonomics.</a> International Journal of Molecular Sciences. 19 (11), 3506 (2018).</li><li>Li, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+insight+into+current+advances+on+pharmacology,+pharmacokinetics,+toxicity+and+detoxification+of+aconitine.">An insight into current advances on pharmacology, pharmacokinetics, toxicity and detoxification of aconitine.</a> Biomedicine &amp; Pharmacotherapy. 151, 113115 (2022).</li><li>Xie, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimization+of+processing+technology+of+braised+Rehmanniae+Raidx+based+on+multiple+indexes+and+response+surface+technology+and+correlation+between+components+and+color.">Optimization of processing technology of braised Rehmanniae Raidx based on multiple indexes and response surface technology and correlation between components and color.</a> Journal of Chinese Traditional Medicine. 47 (18), 4927-4937 (2022).</li><li>Yang, X. Q., Xu, W., Xiao, C. P., Sun, J., Feng, Y. Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+on+processing+technology+of+Atractylodes+chinensis+with+rice+water+and+its+pharmacodynamics+of+anti-diarrhea.">Study on processing technology of Atractylodes chinensis with rice water and its pharmacodynamics of anti-diarrhea.</a> Chinese Herbal Medicines. 53 (1), 78-86 (2022).</li></ol>

TBC is an important Tibetan medicine with the effects of dispelling cold and relieving pain. It has been mostly used to treat traumatic injury and rheumatic arthralgia in China for thousands of years24,25,26. Diterpenoid alkaloids are both active and toxic ingredients of TBC27,28,29. The main toxic effects of the aconitum alkaloids of T...

The dried root of Aconitum pendulum Busch and A. flavum Hand.-Mazz., one of the most famous Tibetan medicines, is called Tiebangchui (TBC) in Chinese1,2. The dried roots of TBC are helpful in dispelling cold and wind, reducing pain, and calming shock. It was recorded in the first volume of "Drug Standards (Tibetan Medicine) of the Ministry of Health of the People's Republic of China," which states that the dried roots of TBC are commonly used to treat rheumatoid arthritis, bruises, and other cold diseases3. However, the clinical therapeutic dose of TBC is similar to its toxic dose, and incidents of poisoning or death have been frequently reported due to improper use4. Therefore, reducing the toxicity and preserving the efficacy of TBC has become a research hot spot over the years.
In Tibetan medicine, processing is one of the most effective methods to attenuate the toxicity of TBC. According to "Processing specification of Tibetan medicine of Qinghai Province (2010)", the original herbs (TBC) should be placed in an iron pot and stir-fried with Zanba until the Zanba turns yellow, after which Zanba is removed and the herbs are dried in air5,6. However, no specific process parameters have been documented, which makes controlling the processing technology and the quality of Zanba-stir-fried TBC difficult. The CRITIC method is an objective weight method that can avoid fuzzification and subjectivity, and enhance the objectivity of weighing7. The Box-Behnken response surface method can directly reflect the interaction between each factor through polynomial fitting8. The combination of the Box-Behnken response surface and CRITIC method is commonly used to optimize processing technology to acquire the optimized processing protocol9,10. In this paper, a monoester-diterpenoid alkaloid (MDA) (benzoylaconitine) and two diester-diterpenoid alkaloids (DDAs) (aconitine, 3-deoxyaconitine) were used as evaluation indexes. CRITIC combined with the Box-Behnken response surface method was applied to optimize the processing technology of Zanba-stir-fried TBC and establish a standard processing method for clinical safe use.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>3-Deoxyaconitine</td>
			<td>Chengdu Desite Biotechnology Co., Ltd.</td>
			<td>DST221109-033</td>
			<td></td>
		</tr>
		<tr>
			<td>Aconitine</td>
			<td>Chengdu Desite Biotechnology Co., Ltd.</td>
			<td>DSTDW000602</td>
			<td></td>
		</tr>
		<tr>
			<td>Ammonium acetate</td>
			<td>Tianjin Kermel Chemical Reagent Co., Ltd</td>
			<td>Chromatographic grade</td>
			<td></td>
		</tr>
		<tr>
			<td>Benzoylaconitine</td>
			<td>Chengdu Desite Biotechnology Co., Ltd.</td>
			<td>DSTDB005502</td>
			<td></td>
		</tr>
		<tr>
			<td>Design-Expert software</td>
			<td>Stat-Ease, Inc., Minneapolis, MN, USA</td>
			<td>version 13.0</td>
			<td></td>
		</tr>
		<tr>
			<td>Electronic analytical balance</td>
			<td>Shanghai Liangping Instruments Co., Ltd.</td>
			<td>FA1004</td>
			<td></td>
		</tr>
		<tr>
			<td>High performance liquid chromatography</td>
			<td>SHIMADZU Co., Ltd.</td>
			<td>LC-20A</td>
			<td></td>
		</tr>
		<tr>
			<td>High-speed smashing machine</td>
			<td>Beijing Zhongxing Weiye Instrument Co., Ltd.</td>
			<td>FW-100</td>
			<td></td>
		</tr>
		<tr>
			<td>Millipore filter</td>
			<td>Tianjin Jinteng Experimental Equipment Co., Ltd</td>
			<td>&#966;13 0.22 Nylon66</td>
			<td></td>
		</tr>
		<tr>
			<td>stir-Fry machine</td>
			<td>Changzhou Maisi Machinery Co., Ltd</td>
			<td>Type 5</td>
			<td></td>
		</tr>
		<tr>
			<td>Tiebangchui</td>
			<td>Gannan Baicao Biotechnology Development Co., Ltd</td>
			<td>20211012</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultra pure water systemic</td>
			<td>RephiLe Bioscience, Ltd.</td>
			<td>Genie G</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultrasonic cleansing machine</td>
			<td>Ningbo Xinyi Ultrasonic Equipment Co., Ltd</td>
			<td>SB2200</td>
			<td></td>
		</tr>
		<tr>
			<td>Zanba</td>
			<td>27 Chuanzang Road, Ganzi County</td>
			<td>-</td>
			<td></td>
		</tr>
	</tbody>
</table>

optimization of processing technology for tiebangchui with zanba based on critic combined with box-behnken response surface method

The dried root of Aconitum pendulum Busch., called Tiebangchui (TBC) in Chinese, is one of the most famous Tibetan medicines. It is a widely used herb in northwest China. However, many cases of poisoning have occurred because of TBC's intense toxicity and because its therapeutic and toxic doses are similar. Therefore, finding a safe and effective method to reduce its toxicity is an urgent task. A search through the Tibetan medicine classics shows that the processing method of TBC stir-fried with Zanba was recorded in the "Processing specification of Tibetan medicine of Qinghai Province (2010)". However, the specific processing parameters are not yet clear. Thus, this study aims to optimize and standardize the processing technology of Zanba-stir-fried TBC.
First, a single-factor experiment was conducted on four factors: the slice thickness of TBC, amount of Zanba, processing temperature, and time. With monoester and diester alkaloid contents in Zanba-stir-fried TBC as indexes, CRITIC combined with the Box-Behnken response surface method was used to optimize the processing technology of Zanba-stir-fried TBC. The optimized processing conditions of Zanba-stir-fried TBC were a TBC slice thickness of 2 cm, three times more Zanba than TBC, a processing temperature of 125 &#176;C, and 60 min of stir-frying. This study determined the optimized and standard processing conditions for the usage of Zanba-stir-fried TBC, thus providing an experimental basis for the safe clinical use and industrial production of Zanba-stir-fried TBC.

Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method

In this study, the elution gradient used had a good resolution (Figure 1) for the three index components in Zanba-stir-fried TBC, as determined after repeated debugging. The three index components in Zanba-stir-fried TBC had a good linear relationship within a specific concentration range (Table 2). The precision (Table 5), stability (Table 6), repeatability (Table 7), and sample recovery (Table 8) of Zanba-...

Watch this Scientific Journal Video about Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method at JoVE.com

Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method (Video) | JoVE 

The present protocol describes an efficient and standard detoxification processing method for Zanba-stir-fried Tiebangchui using CRITIC combined with the Box-Behnken response surface method.

The dried root of Aconitum pendulum Busch., called Tiebangchui (TBC) in Chinese, is one of the most famous Tibetan medicines. It is a widely used herb in ...