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Optical Mapping of Langendorff-perfused Rat Hearts

20.6K Views

11:48 min

August 11th, 2009

August 11th, 2009

20,597 Views

0:05

Title

0:11

Introduction

0:41

Preparing the Solutions and the Langendorff System

2:01

Harvesting of the Rat Heart and Set-up of the Langendorff Perfusion System

4:55

Loading Potentiometric Dye and Acquiring Data

8:45

Analyze Acquisition Information Using RedShirt Imaging Software

10:38

Representative Visualization of a Myocardial Excitation Wave

11:08

Conclusion

This article describes a high temporal and spatial resolution technique to optically image action potential movement on the surface of Langendorff-perfused rat hearts using a potentiometric dye (di-8-ANEPPS).

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