June 27th, 2012
•Bisulfite mutagenesis is the gold standard for analyzing DNA methylation. Our modified protocol allows for DNA methylation analysis at the single-cell level and was specifically designed for individual oocytes. It can also be used for cleavage-stage embryos.
Vidéos Connexes
Homemade Site Directed Mutagenesis of Whole Plasmids Video (Video) | JoVE
An Introduction to Worm Lab: from Culturing Worms to Mutagenesis Video (Video) | JoVE
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli Video (Video) | JoVE
Mouse Oocyte Microinjection, Maturation and Ploidy Assessment Video (Video) | JoVE
Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4 Video (Video) | JoVE
The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry Video (Video) | JoVE
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis Video (Video) | JoVE
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution Video (Video) | JoVE
A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing Video (Video) | JoVE
Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation Video (Video) | JoVE