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Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice Video (Video) | JoVE

DOI :

10.3791/50889-v

October 19th, 2013

October 19th, 2013

17,746 Views

1Department of Pediatrics, Northwestern University Feinberg School of Medicine

We have developed a novel and reproducible technique to isolate primary cultures of pulmonary artery smooth muscle cells (PASMC) from mice as young as P7, thereby allowing better study of the signaling pathways involved in neonatal smooth muscle cell contraction and relaxation.

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