June 21st, 2014
•Here, we describe a protocol based on epigenetic reprogramming of human embryonic stem cells (hESCs) toward generating a homogeneous population of skeletal muscle progenitors that under permissive culture conditions form three-dimensional clusters of contractile myofibers (myospheres), which recapitulate biological features of human skeletal muscles.
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From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs Video (Video) | JoVE
From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel Video (Video) | JoVE
Generation of Single-Cell Suspensions from Mouse Neural Tissue Video (Video) | JoVE
Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System Video (Video) | JoVE
Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set Video (Video) | JoVE
Generation of Transgenic C. elegans by Biolistic Transformation Video (Video) | JoVE
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses Video (Video) | JoVE
In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors Video (Video) | JoVE
Generation of Transgenic Hydra by Embryo Microinjection Video (Video) | JoVE
Targeted DNA Methylation Analysis by Next-generation Sequencing Video (Video) | JoVE