January 23rd, 2019
•This protocol describes the removal and purification of pulmonary artery endothelial cells (PAECs) that adhere to the balloon tips of Swan-Ganz catheters while they are wedged in the pulmonary artery during right heart catheterization and remain adherent to the deflated balloon following removal from the patient's body.
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Vidéos Connexes
Basic Surgical Techniques in the Göttingen Minipig: Intubation, Bladder Catheterization, Femoral Vessel Catheterization, and Transcardial Perfusion Video (Video) | JoVE
Echocardiographic Assessment of the Right Heart in Mice Video (Video) | JoVE
Cardiac Catheterization in Mice to Measure the Pressure Volume Relationship: Investigating the Bowditch Effect Video (Video) | JoVE
Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting Video (Video) | JoVE
Isolation and Culture Expansion of Tumor-specific Endothelial Cells Video (Video) | JoVE
Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins Video (Video) | JoVE
Shunt Surgery, Right Heart Catheterization, and Vascular Morphometry in a Rat Model for Flow-induced Pulmonary Arterial Hypertension Video (Video) | JoVE
Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery Video (Video) | JoVE
The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart Video (Video) | JoVE
Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta Video (Video) | JoVE