Transfection of HEK293T Cells with Dual Color Reporter Construct
2:01
Treatment of Transfected HEK293T Cells with Latency Reversing Agents
2:33
Staining of Transfected Cells with Fixable Viability Dye for Flow Cytometry Analysis
4:40
EGFP and DsRed Measurements by Flow Cytometry and Data Analysis
5:49
Results: Assessment of Latency Reversing Agents on HIV Transcription and Splicing
6:43
Conclusion
Transcription
The overall goal of this procedure is to assess in vitro, the impact of latency reversing agents on HIV mRNA processing. The protocol provide a simple efficient and reliable method for assessing, simultaneously the effect of LRA on HIV transcripti
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A high throughput protocol for functional assessment of HIV efficient reactivation and clearance of latent proviruses is described and applied by testing the impact of interventions on HIV transcription and splicing. Representative results of the effect of latency reversing agents on LTR-driven transcription and splicing are provided.