Preparation of Arabidopsis thaliana Seedlings on a Plastic Mesh
1:36
Colonization of Plants in Liquid Bacterial Growth Medium
3:16
Bacterial Colonization Maintenance
4:14
Collection of Bacteria for Viable Cell Counts
5:19
Collection of Intact Plant Roots for Microscopy
6:13
Results: Assessment of Bacterial Colonization on Arabidopsis thaliana Roots
8:39
Conclusion
Transcription
Most experiments study plant-microbe interactions by focusing on colonization, but our protocol is designed to look both at colonization and maintenance of the bacteria on the root. We can change the experiment to fit different bacteria and plants
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Here we describe a hydroponic plant growth assay to quantify species presence and visualize the spatial distribution of bacteria during initial colonization of plant roots and after their transfer into different growth environments.