In Vitro Macrophage Attraction Assay: Preparation of the Conditioned Media
4:55
In Vitro Macrophage Attraction Assay: Preparation of MV-4-11 Cells
6:03
In Vitro Macrophage Attraction Assay: Transwell Assay
7:25
Results: Analysis of the Assay and its Ability to Study Tumor-macrophage Interaction
8:02
Conclusion
Transcription
Tumor-associated macrophages play important roles in the tumor microenvironment. Understand how different genetic mutations in the tumor cells affect macrophage recruitment is critical for designing personalized treatment for cancer patients. This
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This article represents a useful in vitro assay to evaluate the capability of conditioned medium from tumor cells to attract macrophages.