Liposome Size and Zeta Potential Characterization and Entrapment Efficiency and Drug Loading Calculation
4:11
Preparation and Liposome Injection
6:47
Confocal Imaging and Image Analysis
9:03
Results: Representative Effects of Drug-loaded Lipsome Injection
10:48
Conclusion
Transcription
This protocol describes a technique for targeting drugs of interest to larval zebrafish macrophages to manipulate and evaluate macrophage function. This technique has advantages over traditional drug delivery strategies such as immersion by ensuri
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Here, we describe the synthesis of drug-loaded liposomes and their microinjection into larval zebrafish for the purpose of targeting drug delivery to macrophage-lineage cells.