S'identifier

JoVE Journal

Developmental Biology

This content is Open Access.

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

5.1K Views

08:37 min

October 9th, 2020

October 9th, 2020

5,147 Views

0:04

Introduction

1:00

Embryo Dissection

1:51

Embryo Dissociation and Cell Sorting

3:11

Size Selection

6:02

Results: Small RNA Libraries

7:53

Conclusion

Transcription

We describe a technique for profiling microRNAs in early mouse embryos. This protocol overcomes the challenge of low cell input and small RNA enrichment. This assay can be used to analyze changes in miRNA expression over time in different cell lineages of the early mouse embryo.

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