Casting the Silicone Window and Preparing the Mouse for Insertion of the Silicone Window
1:44
Inserting a Lateral Window for Imaging in the Liver and the Pancreas
3:51
Inserting the Window for Imaging in the Mammary Gland
4:56
Imaging Through the Window
6:04
Results: Investigation of the Adaptability of the PDMS Window to Diverse Tissue Sites
7:05
Conclusion
Transcription
This procedure for casting and inserting silicone windows lowers the technical barriers to longitudinal intravital imaging. Our protocol produces sturdy, easily adaptable windows that are well-tolerated by the animals. Using this technique, we hav
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An approach is here presented for long-term intravital imaging using optically clear, silicone windows that can be glued directly to the tissue/organ of interest and the skin. These windows are cheaper and more versatile than others currently used in the field, and the surgical insertion causes limited inflammation and distress to the animals.